Experimental transmission of Bovine leukemia virus in cattle via rectal palpation.

Abstract

We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV-negative steers. Three of four steers developed antibodies against BLV as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experiments demonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection.

• Authors: Junko Kohara, Satoru Konnai, Misao Onuma
• Journal: The Japanese journal of veterinary research (Jpn J Vet Res) Vol. 54 Issue 1 Pg. 25-30 (May 2006) ISSN: 0047-1917 [Print] Japan
• PMID: 16786975 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• DNA, Viral

Topics

• Animals
• Antibodies, Viral (blood)
• Cattle
• DNA, Viral (chemistry, genetics)
• Digital Rectal Examination (adverse effects, methods)
• Disease Transmission, Infectious (veterinary)
• Enzootic Bovine Leukosis (transmission, virology)
• Female
• Immunodiffusion (veterinary)
• Leukemia Virus, Bovine (genetics, growth & development)
• Male
• Polymerase Chain Reaction (veterinary)