Abstract | OBJECTIVE: METHODS: Twenty eight SD rats were randomly divided into 3 groups, including control group (8 rats), LPS shock group (10 rats), and LPS + VIP group (10 rats). Endotoxic shock model was established by administration of a single dose of 10 mg/kg LPS in LPS shock group, a bolus of 5 nmol VIP intravenous injection following LPS in LPS + VIP group. The rats in the control group were given the same volume of normal saline injection. Blood samples were taken at time points of 1, 2, 4, and 6 hours after intervention from each group for measuring the level of TNF-alpha, IL-1beta and IL-10 by ELISA. Pathological changes of the intestine were observed by light microscope and electron microscope at the animals death or at the end of the experiment. RESULTS: Serum TNF-alpha, IL-1beta and IL-10 levels elevated at each time point in LPS shock group and LPS + VIP group (P < 0.05 or P < 0.01). TNF-alpha concentration reached the peak level 2 h after LPS injection; IL-1beta and IL-10 increased continuously till the end of the experiment. In LPS + VIP group, TNF-alpha and IL-1beta elevated slightly and IL-10 increased significantly as compared with LPS shock group (P < 0.01). Leukocyte infiltration, ischemia, segmental hemorrhage or necrosis appeared in intestine under light microscope and cell swelling, cytoplasmic vacuoles and organelle damage were observed under electron microscope. However, pathological changes in LPS + VIP group were milder than those in LPS group. CONCLUSIONS: VIP improved endotoxic shock-associated histopathological alteration of intestine, down-regulated pro-inflammatory cytokines production and up-regulated anti-inflammatory cytokines. These effects may suggest a protective mechanism of VIP in septic shock. VIP is a potential immunoregulatory substance in treatment of septic shock.
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Authors | Yu-cai Zhang, Li-ping Yang, Ding-hua Tang, Yu-ming Zhang |
Journal | Zhonghua er ke za zhi = Chinese journal of pediatrics
(Zhonghua Er Ke Za Zhi)
Vol. 44
Issue 5
Pg. 369-73
(May 2006)
ISSN: 0578-1310 [Print] China |
PMID | 16780716
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Biomarkers
- Interleukin-1beta
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- Interleukin-10
- Vasoactive Intestinal Peptide
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Topics |
- Animals
- Biomarkers
(blood)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Female
- Interleukin-10
(blood)
- Interleukin-1beta
(blood)
- Intestines
(drug effects, pathology, ultrastructure)
- Lipopolysaccharides
(toxicity)
- Male
- Rats
- Rats, Sprague-Dawley
- Shock, Septic
(blood, drug therapy, immunology)
- Tumor Necrosis Factor-alpha
(blood)
- Vasoactive Intestinal Peptide
(administration & dosage, immunology, pharmacology)
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