[Clonality study of palmar fibromatosis].

Abstract

OBJECTIVE:

To study the clonality of palmar fibromatosis by molecular genetic analysis of X chromosome inactivation pattern at a polymorphic site of human androgen receptor gene (HUMARA).

METHODS:

Twelve female cases of palmar fibromatosis were enrolled into this study. Hematoxylin and eosin-stained sections of paraffin-embedded, formalin-fixed tissues were microdissected by laser capture microdissection technology in order to obtain the proliferative spindle cells. Tumor cells isolated from rectal adenocarcinoma in a female patient were used as positive control. The genomic DNAs were extracted with phenol and chloroform, digested with methylation-sensitive restriction endonuclease HpaII, and amplified by polymerase chain reaction (PCR) using primers targeted to a highly polymorphic short tandem repeat of HUMARA. The amplimers were separated on vertical 8% non-denaturing polyacrylamide gels and the patterns were visualized with ethidium bromide stain.

RESULTS:

The methodology for clonality analysis was validated in the positive control using rectal adenocarcinoma cells. Among the 12 cases studied, PCR amplification failed in 3 samples and 1 sample showed homozygosity which was not suitable for further analysis. Eight samples were successfully amplified and showed a random X chromosome inactivation pattern, suggesting polyclonality in these lesions.

CONCLUSIONS:

Palmar fibromatosis is a polyclonal condition and should be considered as a form of non-neoplasmic fibroblastic proliferation.

• Authors: Lei Wang, Hong-guang Zhu
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 35 Issue 4 Pg. 224-7 (Apr 2006) ISSN: 0529-5807 [Print] China
• PMID: 16776980 (Publication Type: English Abstract, Journal Article)

Chemical References

• ACTA2 protein, human
• AR protein, human
• Actins
• Receptors, Androgen

Topics

• Actins (metabolism)
• Adolescent
• Adult
• Aged
• Child
• Chromosomes, Human, X
• Clone Cells (metabolism, pathology)
• Dupuytren Contracture (genetics, metabolism, pathology)
• Female
• Humans
• Middle Aged
• Polymerase Chain Reaction
• Receptors, Androgen (genetics, metabolism)
• X Chromosome Inactivation