Protective effects of trapidil in ischemia-reperfusion injury due to testicular torsion and detorsion: an experimental study.

Abstract

OBJECTIVE:

We aimed to detect the preventive effects of trapidil in ischemia-reperfusion (IR) injury due to testicular torsion and detorsion.

METHODS:

Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose 1 h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination.

RESULTS:

The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P<0.05, P<0.001 and P<0.001, respectively). A statistical difference was not found among study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P>0.05). The T-SH level in the study group was significantly higher than in the IR, sham and sham plus trapidil groups in left testis P<0.05). In the IR group (left testis), grade 1 interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade 1 interstitial injury was 30% (3/10) and there was no injury in 70% (7/10).

CONCLUSION:

Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.

• Authors: Salih Somuncu, Murat Cakmak, Sibel Erdogan, Osman Caglayan, Hülya Akman, Murat Kaya
• Journal: International journal of urology : official journal of the Japanese Urological Association (Int J Urol) Vol. 13 Issue 5 Pg. 601-5 (May 2006) ISSN: 0919-8172 [Print] Australia
• PMID: 16771732 (Publication Type: Journal Article)

Chemical References

• Sulfhydryl Compounds
• Nitric Oxide
• Malondialdehyde
• Trapidil

Topics

• Animals
• Disease Models, Animal
• Male
• Malondialdehyde (metabolism)
• Nitric Oxide (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (etiology, metabolism, pathology, prevention & control)
• Spermatic Cord Torsion (complications, drug therapy, metabolism, pathology)
• Sulfhydryl Compounds (metabolism)
• Trapidil (pharmacology)