Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes.

Abstract

Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 microg/ml) and EA/EtOH (25-75 microg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.

• Authors: Hsin-Ling Yang, Ssu-Ching Chen, Nai-Wen Chang, Jia-Ming Chang, Mei-Ling Lee, Pei-Chuan Tsai, Han-Hsuan Fu, Wei-Wan Kao, Hsiao-Chi Chiang, Hsuan-Hui Wang, You-Cheng Hseu
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 44 Issue 9 Pg. 1513-21 (Sep 2006) ISSN: 0278-6915 [Print] England
• PMID: 16765500 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Antioxidants
• Drugs, Chinese Herbal
• Membrane Proteins
• Ethanol
• ethyl acetate
• Adenosine Triphosphate
• Superoxide Dismutase
• Glutathione

Topics

• Acetates (chemistry)
• Adenosine Triphosphate (metabolism)
• Antioxidants (pharmacology)
• Bidens (chemistry)
• Cells, Cultured
• Cytosol (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Electrophoresis, Polyacrylamide Gel
• Erythrocyte Membrane (drug effects, metabolism)
• Erythrocytes (drug effects, metabolism)
• Ethanol (chemistry)
• Glutathione (metabolism)
• Humans
• Membrane Proteins (chemistry)
• Oxidative Stress (drug effects)
• Superoxide Dismutase (metabolism)