Immunohistochemical detection of MT1-MMP, RECK, and EMMPRIN in ameloblastic tumors.

Abstract

BACKGROUND:

To evaluate the roles of matrix-degrading proteinase regulators in progression of odontogenic tumors, expression of membrane-bound matrix metalloproteinase (MMP) MT1-MMP, MMP inhibitor RECK and MMP inducer EMMPRIN was analyzed in ameloblastic tumors as well as in tooth germs.

METHODS:

Tissue specimens of 11 tooth germs, 40 ameloblastomas, and five malignant ameloblastic tumors were examined immunohistochemically with the use of antibodies against MT1-MMP, RECK, and EMMPRIN.

RESULTS:

Immunohistochemical reactivity for MT1-MMP, RECK and EMMPRIN was detected predominantly in odontogenic epithelial cells near the basement membrane in tooth germs and benign and malignant ameloblastic tumors. The level of immunoreactivity for MT1-MMP was slightly higher in benign and malignant ameloblastic tumors than in tooth germs. RECK expression was lower in ameloblastomas than in tooth germs. Follicular ameloblastomas showed significantly lower expression of RECK than plexiform ameloblastomas, and immunoreactivity for RECK in acanthomatous ameloblastomas was slightly lower than that in other cellular variants.

CONCLUSION:

Expression of MT1-MMP, RECK and EMMPRIN in tooth germs and ameloblastic tumors suggests that these normal and neoplastic epithelial components control MMP-dependent extracellular matrix (ECM) degradation during tooth development and tumor progression via epithelial-mesenchymal interactions.

• Authors: H Kumamoto, K Ooya
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 35 Issue 6 Pg. 345-51 (Jul 2006) ISSN: 0904-2512 [Print] Denmark
• PMID: 16762015 (Publication Type: Journal Article)

Chemical References

• GPI-Linked Proteins
• Membrane Glycoproteins
• RECK protein, human
• Basigin
• Matrix Metalloproteinases
• Matrix Metalloproteinases, Membrane-Associated

Topics

• Ameloblastoma (chemistry, enzymology)
• Basigin (analysis)
• GPI-Linked Proteins
• Humans
• Immunohistochemistry
• Jaw Neoplasms (chemistry, enzymology)
• Matrix Metalloproteinases (analysis)
• Matrix Metalloproteinases, Membrane-Associated
• Membrane Glycoproteins (analysis)
• Tooth Germ (chemistry, enzymology)