[Experimental study of anti-cancer effect of Mannan-modified targeted nanoliposome on mice].

Abstract

OBJECTIVE:

To test the anti-cancer effect of mannan-modified targeted nanoliposome on mice.

METHODS:

The 3beta[N-(N',N'-dimethylaminoethane) -carbamoyl] cholesterol (DC-chol) was synthesized by the chemical reaction of cholesteryl chloroformate and N, N-dimethylethylendiamine. The DC-chol was then mixed with dioleoylphosphatidylethanolamine (DOPE) and chol-AECM-mannan to produce the sonicated mannan-modified targeted nanoliposome. The size of the nanoliposome was measured by zetasizer. Nanoliposome or purified EGFR (as control) was injected to the C57 mice with implanted lung cancers intravenously. The growth of tumors was observed, followed by ELISA and Western tests of serum antibodies.

RESULTS:

The liposome nanoparticle had a size of 132 nm. The experimental groups had significant higher levels of blood serum antibodies than the controls. The experimental groups also had less volumes of tumors than the controls (P<0.05).

CONCLUSION:

The growth of tumor has been inhibited by mannan-modified targeted nanoliposome. The mannan-modified targeted nanoliposome has anti-cancer effect on mice.

• Authors: Li Yang, Li Cheng, Ling Tian, Lin-yu Fan, Hong-mei Yin, Yu-quan Wei
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 37 Issue 3 Pg. 357-60 (May 2006) ISSN: 1672-173X [Print] China
• PMID: 16761407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drug Carriers
• Liposomes
• Mannans
• N-(2-(cholesteryloxycarbonylamino)ethyl)carbamoylmethylated mannan
• Cholesterol
• ErbB Receptors

Topics

• Animals
• Antineoplastic Agents (administration & dosage, pharmacology)
• Cholesterol (administration & dosage, analogs & derivatives, pharmacology)
• Drug Carriers
• Drug Delivery Systems
• ErbB Receptors (administration & dosage)
• Female
• Liposomes (administration & dosage)
• Lung Neoplasms (drug therapy)
• Mannans (administration & dosage, pharmacology)
• Mice
• Mice, Inbred C57BL
• Nanoparticles
• Neoplasm Transplantation
• Random Allocation