Detection of non-maternal components of gestational choriocarcinoma by PCR-based microsatellite DNA assay.

Abstract

OBJECTIVE:

Gestational and non-gestational choriocarcinomas have distinctly different tissues of origin, parental genotypes, natural histories, and responses to therapy. Our objective was to develop a convenient, fast, and reliable assay that would, using only patient tissue, allow separation of gestational from non-gestational choriocarcinomas.

METHOD:

Benign and malignant tissues, preserved in paraffin blocks and separated by microdissection, were examined using a commercial PCR-based tissue identity assay (ABI AmpFlSTR Profiler Plus Kit and ABI 377 DNA sequencer) to detect genetic profiles of 9 microsatellite markers, along with X and Y chromosome markers. Cases included 6 choriocarcinomas. Controls included eight non-germ cell reproductive tract tumors and two hydatidiform moles.

RESULTS:

The microsatellite markers identified the five choriocarcinomas diagnosed on clinical and histological grounds as gestational, to be of genetically non-maternal (androgenic) origin. The neoplasm previously classified as a non-gestational choriocarcinoma was demonstrated to be of maternal origin, as were the non-germ cell reproductive tract tumors. Samples from hydatidiform moles contained either androgenic markers only or a mix of maternal and androgenic markers, as previously seen in complete and partial moles, respectively.

CONCLUSION:

A commercially available microsatellite DNA diagnostic assay is a quick and convenient way to discriminate between gestational and non-gestational choriocarcinoma.

• Authors: Milena Cankovic, Arthur R Gaba, Frederick Meier, Wooshin Kim, Richard J Zarbo
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 103 Issue 2 Pg. 614-7 (Nov 2006) ISSN: 0090-8258 [Print] United States
• PMID: 16740299 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm

Topics

• Alleles
• Case-Control Studies
• Choriocarcinoma (genetics)
• Choriocarcinoma, Non-gestational (genetics)
• DNA, Neoplasm (genetics)
• Fallopian Tube Neoplasms (genetics)
• Female
• Humans
• Lung Neoplasms (genetics)
• Microsatellite Repeats (genetics)
• Ovarian Neoplasms (genetics)
• Polymerase Chain Reaction (methods)
• Pregnancy
• Uterine Neoplasms (genetics)