Quantitative analysis of cyclin E and protein p34 cdc2 expression in superficial bladder cancer.

Abstract

In the present study, the expression of cyclin E and kinase p34 cdc2 was investigated in preinvasive bladder tumors. The study material consisted of bladder sections (grades: GI--16 cases, GII--10, and GIII--12) collected from 38 patients in the course of the tumor electroresection. Immunohistochemical examinations were carried out with immunoperoxidase method. Antigens were labeled with NCL-CYCLIN E or NCL-p34 cdc2 monoclonal antibodies (Novocastra, UK). Positive reaction was demonstrated using ABC-universal Kit (Novocastra, UK). Differences in the protein expression in relation to the tumor grade were determined with a non-parametric Mann-Whitney's test. Increasing grade of tumors was associated with down regulation of cyclin E visible as lower percentage of cyclin E-positive cells. These changes were statistically significant for GI group as compared to groups GII and GIII (p<0.001). There were no differences between the study groups in the p34 protein expression. Cyclin E expression was inversely correlated with tumor grade therefore may be helpful in establishing therapeutic procedure.

• Authors: Piotr Kaczmarek, Dariusz Tosik, Ewa Majewska, Zbigniew Baj
• Journal: Polish journal of pathology : official journal of the Polish Society of Pathologists (Pol J Pathol) Vol. 57 Issue 1 Pg. 41-4 ( 2006) ISSN: 1233-9687 [Print] Poland
• PMID: 16739882 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Cyclin E
• CDC2 Protein Kinase

Topics

• Aged
• Biomarkers, Tumor (metabolism)
• CDC2 Protein Kinase (metabolism)
• Carcinoma, Transitional Cell (metabolism, pathology, surgery)
• Cyclin E (metabolism)
• Down-Regulation
• Female
• Humans
• Male
• Middle Aged
• Neoplasm Staging
• Urinary Bladder Neoplasms (metabolism, pathology, surgery)