Abstract | BACKGROUND/AIMS: METHODS: 50 male Sprague-Dawley rats weighing 255 +/- 39 g were housed. Forty rats were injected 6 mg/kg adriamycin into tail veins under anesthesia to induce nephrosis, while 10 rats were spared as sham control. After the stabilization of proteinuria at the sixth week, the rats were treated for 6 weeks by losartan (n = 10, 30 mg/kg/day), HBO (n = 10, 2.8 atmosphere absolute, 90 min/day), HBO + losartan (n = 10) and vehicle (n = 10). Protein carbonyl (PCO), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analyzed from tissue specimens. Biochemical markers were studied from venous samples and 24-hour urine was collected for proteinuria. The surviving animals at 12 weeks (vehicle group (n = 6), HBO (n = 6), losartan (n = 8), HBO + losartan (n = 10) were sacrificed. Glomerular sclerosis, tubulointerstitial and blood vessel changes were determined by semiquantitative scoring. RESULTS: The PCO levels increased (p < 0.001), and the GPx and SOD levels decreased (p < 0.001 for both) in the nephrotic rats. In losartan and HBO groups GPx levels increased (p = 0.001, p = 0.002 respectively), but PCO and SOD levels did not change. The combination of HBO with losartan significantly increased the GPx and SOD levels (p = 0.001 for both) and decreased PCO levels (p = 0.005). HBO but not losartan significantly reduced proteinuria (p < 0.001). The combination of HBO and losartan reduced proteinuria better than the single losartan regime (p < 0.001). The effect of the combination was also noticed on the histological examination of the kidneys. The activities, appetites, weight gains, and improvement of edema were better in the HBO combined with losartan regime. CONCLUSIONS:
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Authors | Mahmut Ilker Yilmaz, Ahmet Korkmaz, Ayper Kaya, Alper Sonmez, Kayser Caglar, Turgut Topal, Tayfun Eyileten, Mujdat Yenicesu, Cengizhan Acikel, Sukru Oter, Halil Yaman, Huseyin Aktug, Yusuf Oguz, Abdulgaffar Vural, T Alp Ikizler |
Journal | Nephron. Experimental nephrology
(Nephron Exp Nephrol)
Vol. 104
Issue 1
Pg. e15-22
( 2006)
ISSN: 1660-2129 [Electronic] Switzerland |
PMID | 16699289
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2006 S. Karger AG, Basel. |
Chemical References |
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Topics |
- Animals
- Doxorubicin
- Drug Synergism
- Hyperbaric Oxygenation
- Kidney
(drug effects, pathology)
- Losartan
(therapeutic use)
- Male
- Nephrotic Syndrome
(chemically induced, drug therapy, pathology)
- Oxidative Stress
(drug effects)
- Proteinuria
(chemically induced, drug therapy)
- Rats
- Rats, Sprague-Dawley
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