Abstract | PURPOSE:
Survivin, a member of the inhibitor of apoptosis gene family, is expressed in most common cancers. We investigated the expression pattern of survivin in the tumors of patients with bladder cancer and assessed the diagnostic potential of RT-PCR detection of survivin mRNA in urine. MATERIALS AND METHODS: RT-PCR was used to analyze mRNA expression of survivin in 161 cases of bladder cancer, including TCC in 97, SCC in 53 and adenocarcinoma in 11, and their matched nontumor tissues. Urine specimens (50 ml) were collected from 84 patients in whom bladder cancer was documented by transurethral resection or biopsy, 41 with nonbladder cancer urological diseases and 42 healthy volunteers. Total RNA was extracted from urine sediments and RT-PCR was performed for survivin. RESULTS: CONCLUSIONS:
Survivin mRNA detection in urine sediment using RT-PCR shows high sensitivity and specificity for bladder cancer. It may prove useful for the routine screening and monitoring of patients.
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Authors | Omar Moussa, Hassan Abol-Enein, Nabil K Bissada, Thomas Keane, Mohamed A Ghoneim, Dennis K Watson |
Journal | The Journal of urology
(J Urol)
Vol. 175
Issue 6
Pg. 2312-6
(Jun 2006)
ISSN: 0022-5347 [Print] United States |
PMID | 16697865
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- RNA, Messenger
- Survivin
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Topics |
- Gene Expression Regulation, Neoplastic
- Humans
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
(genetics)
- Neoplasm Proteins
(genetics)
- RNA, Messenger
(urine)
- Reverse Transcriptase Polymerase Chain Reaction
- Survivin
- Urinary Bladder Neoplasms
(diagnosis, genetics, urine)
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