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The model of D-galactosamine-induced injury of rat hepatocytes in primary culture.

Abstract
D-galactosamine (GalN) is a highly selective hepatotoxin that causes liver damage similar to human viral hepatitis via depletion of uridine nucleotides, which subsequently diminishes synthesis of RNA and proteins. Model of galactosamine hepatotoxicity is frequently used in animal experiments in vitro. The purpose of our study was to establish the model of GalN-induced hepatocyte injury in in vitro conditions using primocultures of rat hepatocytes as an important pre-requisite for further experiments in which we would like to study potential hepatoprotective effect of various substances. Rate of hepatocyte injury was evaluated by morphological changes, changes in cell viability, albumin production, mitochondrial membrane potential, activity of mitochondrial dehydrogenases and glutathione content. Marked dose dependent hepatocyte injury was found after 24-hour incubation with GalN. Based on the results we suggest as an optimal model for short-term toxicity test exposure to GalN for 24 hours in dose of 40 mM.
AuthorsOtto Kucera, Halka Lotková, Roman Kand'ár, Renata Hézová, Vladimíra Muzáková, Zuzana Cervinková
JournalActa medica (Hradec Kralove) (Acta Medica (Hradec Kralove)) Vol. 49 Issue 1 Pg. 59-65 ( 2006) ISSN: 1211-4286 [Print] Czech Republic
PMID16696445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Galactosamine
Topics
  • Animals
  • Cells, Cultured
  • Chemical and Drug Induced Liver Injury (etiology, pathology)
  • Disease Models, Animal
  • Galactosamine (toxicity)
  • Hepatocytes (drug effects, metabolism, pathology)
  • Male
  • Rats
  • Rats, Wistar

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