Genetic and epigenetic analysis of the TIMP-3 gene in ovarian cancer.

Abstract

Chromosome 22q shows a high frequency of loss of heterozygosity (LOH) in ovarian cancers suggesting the existence of one or more important tumor suppressor genes (TSGs). The tissue inhibitor of metalloproteinase-3 (TIMP-3) is a plausible TSG candidate since it is often encompassed within these regions of LOH. TIMP-3 has not previously been investigated for somatic mutations or promoter hypermethylation in ovarian cancer. We analyzed 65 ovarian cancers for both somatic genetic mutations and TIMP-3 promoter hypermethylation. Screening of all coding exons of TIMP-3 did not reveal any somatic genetic mutations and only 1/65 showed TIMP-3 methylation. Our data indicate that inactivation of TIMP-3 by somatic mutation or promoter hypermethylation is rare in ovarian cancer.

• Authors: Mira C P Liu, David Y H Choong, Christine S F Hooi, Louise H Williams, Ian G Campbell
• Journal: Cancer letters (Cancer Lett) Vol. 247 Issue 1 Pg. 91-7 (Mar 08 2007) ISSN: 0304-3835 [Print] Ireland
• PMID: 16644110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tissue Inhibitor of Metalloproteinases
• tissue inhibitor of metalloproteinase-4

Topics

• Base Sequence
• Chromosomes, Human, Pair 22
• DNA Methylation
• Female
• Genes, Tumor Suppressor
• Humans
• Loss of Heterozygosity
• Molecular Sequence Data
• Mutation
• Ovarian Neoplasms (genetics)
• Polymorphism, Single-Stranded Conformational
• Promoter Regions, Genetic
• Tissue Inhibitor of Metalloproteinases (genetics)