Functional genomics of PPAR-gamma in human immunomodulatory cells.

Abstract

Keeping in view the fact that peroxisome-proliferators activated receptors-PPARs (alpha,gamma) play a crucial role in atherogenic inflammation, the present study was addressed to explore as to how selective and specific PPAR-gamma gene silencing within human mononuclear cells affects genes involved in lipid metabolism and innate immune process. Such a study revealed that with respect to control cells, the PPAR-gamma knock-out cells exhibited significant reduction in the expression of genes coding for PPAR- alpha, CD-36, LDL-R as well as significant increase in the expression of genes coding for IL-4, IL-8, IFN-gamma, CX3CR1, hTERT. However, the expression of genes coding for LXR-alpha and Receptor-C( k ) could not be detected in PPAR-gamma knock-out cells. Based on these results, we propose that PPAR-gamma gene has the inherent capacity to influence the lipid mediated inflammation process in atherosclerotic lesions.

• Authors: Deepak Kaul, Paras K Anand, Amit Khanna
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 290 Issue 1-2 Pg. 211-5 (Oct 2006) ISSN: 0300-8177 [Print] Netherlands
• PMID: 16633734 (Publication Type: Journal Article)

Chemical References

• PPAR gamma
• RNA, Messenger
• RNA, Small Interfering

Topics

• Cells, Cultured
• Genomics
• Humans
• Immunity, Innate (genetics)
• Inflammation (genetics)
• Lipid Metabolism (genetics)
• PPAR gamma (blood, genetics, physiology)
• RNA, Messenger (metabolism)
• RNA, Small Interfering
• Transfection