Abstract | OBJECTIVE: Angiogenesis is an essential component for tumor development regulated by both proangiogenic and antiangiogenic factors. Thrombospondin 1 (TSP 1) suppresses angiogenesis by inhibiting endothelial cell proliferation and inducing endothelial cell apoptosis. The aim of this study was to compare the expression of TSP 1 in cases with leiomyoma, uterine smooth muscle tumor of uncertain malignant potential (STUMP) and leiomyosarcoma (LMS). Furthermore, we evaluated the prognostic relevance of TSP 1 in uterine LMS. METHODS: TSP 1 expression was investigated by immunohistochemistry from paraffin-embedded tissue in 26 patients with leiomyoma, in 24 patients with STUMP and in 21 patients with LMS. Standard immunohistochemical techniques were used to study the expression of TSP 1 in 5-mum-thick tumor sections. TSP 1 expression was correlated with survival using the Kaplan-Meier method and log-rank test for univariate analysis. RESULTS: TSP 1 was expressed in 77% of leiomyomas, in 13% of STUMP and in 24% of LMS. A statistically significant difference regarding the frequency of TSP 1 expression was observed between leiomyoma and LMS (P < 0.05) as well as between leiomyoma and STUMP (P < 0.05), but not between LMS and STUMP (P > 0.05). Furthermore, a statistically significant correlation between vascular space involvement and TSP 1 expression was observed in patients with uterine LMS, with patients without vascular space involvement having more frequently TSP 1 positive tumors (P = 0.04). No statistically significant correlation between TSP 1 and clinical stage, age and recurrence disease could be detected (P > 0.05). CONCLUSIONS: We found that TSP 1 was more frequently expressed in leiomyoma compared to STUMP and LMS. Additionally, the statistically significant negative correlation between vascular space involvement and TSP 1 expression in patients with uterine LMS shows that TSP 1 might work as a predictive factor in patients with LMS. Further clinical studies are necessary to prove our results and to clarify the role of TSP 1 in uterine smooth muscle tumors.
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Authors | Barbara Bodner-Adler, Andreas Nather, Klaus Bodner, Klaus Czerwenka, Oliver Kimberger, Sepp Leodolter, Klaus Mayerhofer |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 103
Issue 1
Pg. 186-9
(Oct 2006)
ISSN: 0090-8258 [Print] United States |
PMID | 16595146
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Female
- Humans
- Immunohistochemistry
- Leiomyoma
(blood supply, metabolism, pathology)
- Leiomyosarcoma
(blood supply, metabolism, pathology)
- Middle Aged
- Neoplasm Staging
- Neovascularization, Pathologic
(metabolism)
- Retrospective Studies
- Smooth Muscle Tumor
(blood supply, metabolism, pathology)
- Thrombospondin 1
(biosynthesis)
- Uterine Neoplasms
(blood supply, metabolism, pathology)
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