Ki-ras activation in pancreatic carcinomas of Syrian hamsters induced by N-nitrosobis(2-hydroxypropyl)amine.

Abstract

Five pancreatic carcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki-ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki-ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N-nitrosobis(2-oxopropyl)amine.

• Authors: T Ushijima, M Tsutsumi, R Sakai, Y Ishizaka, F Takaku, Y Konishi, M Takahashi, T Sugimura, M Nagao
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 82 Issue 9 Pg. 965-8 (Sep 1991) ISSN: 0910-5050 [Print] Japan
• PMID: 1657847 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• DNA, Neoplasm
• Nitrosamines
• diisopropanolnitrosamine

Topics

• Animals
• Base Sequence
• Carcinogens
• Carcinoma, Intraductal, Noninfiltrating (chemically induced, genetics)
• Cricetinae
• DNA, Neoplasm (genetics)
• Gene Expression Regulation, Neoplastic (drug effects)
• Genes, ras (drug effects)
• Mesocricetus
• Molecular Sequence Data
• Nitrosamines
• Pancreatic Neoplasms (chemically induced, genetics)