Novel single nucleotide polymorphism of UGT1A9 gene in Japanese.

Abstract

We sequenced from 5'-franking region to intron 1 (to 337 bp downstream from exon 1) of the UDP-glucuronosyltransferase (UGT) 1A9 gene prepared from 55 Japanese cancer patients. Seven single nucleotide polymorphisms (SNPs) were found. Two of them were UGT1A9 -118(T)n (n=10) and UGT1A9*5, and four were reported SNPs in intron 1 of UGT1A9 gene (89540C>T, 89549G>A, 89616T>A and 89710A>C). A novel SNP (89587T>C) was found. The sequence is as follows: SNP, 050824FujitaK001; Gene Name, UGT1A9; Accession Number, AF297093; Length, 25 bases; 5'-CCTTCTTGAAGAT/CATGTATTTATAA-3'. Two patients were heterozygous for the mutant allele, resulting in the allele frequency of 1.82%.

• Authors: Ken-ichi Fujita, Yuichi Ando, Fumio Nagashima, Wataru Yamamoto, Hisashi Endo, Keiji Kodama, Kazuhiro Araki, Toshimichi Miya, Masaru Narabayashi, Yasutsuna Sasaki
• Journal: Drug metabolism and pharmacokinetics (Drug Metab Pharmacokinet) Vol. 21 Issue 1 Pg. 79-81 (Feb 2006) ISSN: 1347-4367 [Print] England
• PMID: 16547398 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• DNA, Neoplasm
• UGT1A9 protein, human
• Irinotecan
• Glucuronosyltransferase
• UDP-Glucuronosyltransferase 1A9
• Camptothecin

Topics

• Alleles
• Antineoplastic Agents, Phytogenic (pharmacokinetics, therapeutic use)
• Camptothecin (analogs & derivatives, pharmacokinetics, therapeutic use)
• DNA, Neoplasm (genetics)
• Exons (genetics)
• Gene Frequency
• Glucuronosyltransferase (genetics)
• Humans
• Introns (genetics)
• Irinotecan
• Japan (epidemiology)
• Molecular Sequence Data
• Mutation (genetics)
• Neoplasms (genetics)
• Polymorphism, Single Nucleotide (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• UDP-Glucuronosyltransferase 1A9