Novel single nucleotide polymorphism of UGT1A7 gene in Japanese.

Abstract

We sequenced exon 1 of the UDP-glucuronosyltransferase (UGT) 1A7 gene from 52 Japanese cancer patients. Four single nucleotide polymorphisms (SNPs) were found. Three of them caused UGT1A7*2 and UGT1A7*3. A novel SNP (98973G>C) causing amino acid substitution (Ser141Cys) was found. The sequence is as follows: SNP, 050824FujitaK002; Gene Name, UGT1A7; Accession Number, AF297093; Length, 25 bases; 5'-TAAAGGAGAGTTG/CTTTTGATGCAGT-3'. One out of 52 cancer patients was heterozygous for the variant allele, resulting in the allele frequency of 0.96%. The patient did not possess UGT1A7*2 or UGT1A7*3.

• Authors: Ken-ichi Fujita, Yuichi Ando, Fumio Nagashima, Wataru Yamamoto, Hisashi Endo, Keiji Kodama, Kazuhiro Araki, Toshimichi Miya, Masaru Narabayashi, Yasutsuna Sasaki
• Journal: Drug metabolism and pharmacokinetics (Drug Metab Pharmacokinet) Vol. 21 Issue 1 Pg. 75-8 (Feb 2006) ISSN: 1347-4367 [Print] England
• PMID: 16547397 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Irinotecan
• DNA
• Glucuronosyltransferase
• UDP-glucuronosyltransferase, UGT1A7
• Camptothecin

Topics

• Alleles
• Amino Acid Substitution
• Antineoplastic Agents, Phytogenic (pharmacokinetics, therapeutic use)
• Area Under Curve
• Camptothecin (analogs & derivatives, pharmacokinetics, therapeutic use)
• DNA (genetics)
• Exons (genetics)
• Female
• Gene Frequency
• Glucuronosyltransferase (genetics)
• Humans
• Irinotecan
• Japan (epidemiology)
• Male
• Molecular Sequence Data
• Neoplasms (enzymology)
• Polymorphism, Single Nucleotide (genetics)
• Reverse Transcriptase Polymerase Chain Reaction