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Nuclear factor Nrf2 and antioxidant response element regulate NRH:quinone oxidoreductase 2 (NQO2) gene expression and antioxidant induction.

Abstract
Human NRH:quinone oxidoreductase 2 (NQO2) is a cytosolic protein that catalyzes the metabolic reduction of quinones and provides protection against myelogenous hyperplasia and chemical carcinogenesis. NQO2 gene expression is induced in response to antioxidant tert-butylhydroquinone (tBHQ). Sequence analysis revealed six putative antioxidant response elements (ARE1 through 6) in the human NQO2 gene promoter. Deletion mutagenesis and transfection studies suggested that the ARE region between nucleotides -1433 and -1424 is essential for basal expression and antioxidant induction of NQO2 gene expression. Mutation of this ARE from 3.8 kb NQO2 gene promoter significantly repressed expression and abrogated the induction in response to antioxidant in transfected cells. Band shift, supershift, and chromatin immunoprecipitation (ChIP) assays demonstrated binding of nuclear factors Nrf2 and JunD with human NQO2 gene ARE. Coimmunoprecipitation experiments revealed an association between Nrf2 and JunD. Overexpression of Nrf2 upregulated and overexpression of Nrf2 dominant-negative mutant downregulated ARE-mediated NQO2 gene expression. The treatment of Hep-G2 cells with Nrf2-specific RNAi significantly reduced Nrf2 and NQO2 gene expression and tBHQ induction. The results combined demonstrated that Nrf2 associates with JunD, binds to ARE at nucleotide -1433, and regulates human NQO2 gene expression and induction in response to antioxidants.
AuthorsWei Wang, Anil K Jaiswal
JournalFree radical biology & medicine (Free Radic Biol Med) Vol. 40 Issue 7 Pg. 1119-30 (Apr 01 2006) ISSN: 0891-5849 [Print] United States
PMID16545679 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Antioxidants
  • Hydroquinones
  • JDP2 protein, human
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Repressor Proteins
  • 2-tert-butylhydroquinone
  • NRH - quinone oxidoreductase2
  • Quinone Reductases
Topics
  • Antioxidants (metabolism)
  • Blotting, Northern
  • Cells, Cultured
  • Chromatin Immunoprecipitation
  • Down-Regulation
  • Gene Deletion
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Hydroquinones (pharmacology)
  • Mutagenesis
  • NF-E2-Related Factor 2 (physiology)
  • Promoter Regions, Genetic (genetics)
  • Quinone Reductases (biosynthesis, genetics)
  • Repressor Proteins (physiology)
  • Transfection
  • Up-Regulation

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