Expression profile of mucins (MUC2, MUC5AC and MUC6) in Helicobacter pylori infected pre-neoplastic and neoplastic human gastric epithelium.

Abstract

BACKGROUND:

Helicobacter pylori (H. pylori) causes gastritis and intestinal metaplasia (IM) that may evolve to gastric carcinoma. The objective of this study was to compare the profile of mucins in the progressive stages of H. pylori infected pre-neoplastic and neoplastic human gastric epithelium. We used a panel of monoclonal antibodies with well-defined specificities of MUC2, MUC5AC and MUC6 to characterize the expression pattern of mucins by immunohistochemistry.

METHODS:

RUT and ELISA were down for H. pylori confirmation. Human gastric biopsy sections were stained using immunohistochemistry with MUC2, MUC5AC and MUC6 antibodies.

RESULTS:

MUC5AC was expressed in the superficial epithelium and the upper part of the gastric pits. MUC6 expression was detected in the lower part of the gastric glands. MUC2 was expressed in intestinal metaplasia, mostly in goblet cells. The mucin expression profile in the progressive stages of H. pylori infected human gastric epithelium allows the identification of intestinal metaplasia, which is characterized by a decreased expression of the gastric mucins (MUC5AC and MUC6) and de novo expression of MUC2.

CONCLUSION:

In conclusion, our results suggest that there is altered expression of MUC5AC and MUC6 together with the aberrant expression of MUC2 in intestinal metaplasia, during the process of gastric carcinogenesis. The present study indicates that the MUC2 mucin expression pattern is a reliable marker of intestinal metaplasia, which appears in the context of H. pylori infected individuals.

• Authors: Subramani Durai Babu, Venkataraman Jayanthi, Niranjali Devaraj, Celso A Reis, Halagowder Devaraj
• Journal: Molecular cancer (Mol Cancer) Vol. 5 Pg. 10 (Mar 19 2006) ISSN: 1476-4598 [Electronic] England
• PMID: 16545139 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• MUC2 protein, human
• MUC5AC protein, human
• MUC6 protein, human
• Mucin 5AC
• Mucin-2
• Mucin-6
• Mucins

Topics

• Antibodies, Monoclonal (chemistry)
• Biopsy
• Enzyme-Linked Immunosorbent Assay
• Gastric Mucosa (metabolism)
• Gene Expression Regulation, Neoplastic
• Helicobacter pylori (metabolism)
• Humans
• Immunohistochemistry
• Intestinal Mucosa (pathology)
• Mucin 5AC
• Mucin-2
• Mucin-6
• Mucins (biosynthesis)
• Stomach (microbiology)
• Stomach Neoplasms (metabolism, microbiology)