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Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19.

Abstract
A synthetic peptide corresponding to a part of the virus protein 1-virus protein 2 overlapping region of human parvovirus B19 was used in an indirect enzyme-linked immunosorbent assay. Antibodies of the immunoglobulin (Ig) M class were measured in serum samples from patients with erythema infectiosum and controls. In comparison with an IgM assay using native B19 viral antigen, the peptide antigen assay was 92% sensitive and 87% specific. B19 IgM reactivities were seen in a limited number of children with other viral diseases. Specific IgM reactivities to short synthetic viral peptides have previously been reported only with Epstein-Barr virus. Since other sources of viral antigen are limited, the peptide antigen assay may be a useful alternative for the diagnosis of B19-associated disease in human beings.
AuthorsE Fridell, B J Cohen, B Wahren
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 29 Issue 7 Pg. 1376-81 (Jul 1991) ISSN: 0095-1137 [Print] United States
PMID1653264 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin M
  • Peptides
Topics
  • Amino Acid Sequence
  • Antibodies, Viral (analysis)
  • Antigens, Viral
  • Blood Donors
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Evaluation Studies as Topic
  • Humans
  • Immunoglobulin M (analysis)
  • Molecular Sequence Data
  • Parvoviridae (classification, immunology)
  • Parvoviridae Infections (diagnosis, immunology)
  • Peptides (chemistry, immunology)
  • Sensitivity and Specificity
  • Virus Diseases (immunology)

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