Correlation of human Bub1 expression with tumor-proliferating activity in salivary gland tumors.

Abstract

Human Bub1 plays an important role at the spindle assembly check-point to prevent cell cycle progression following spindle damage. We examined the expression of Bub1 mRNA and protein in 21 human salivary gland tumors (7 pleomorphic adenomas, 2 warthin tumors, 5 mucoepidermoid carcinomas, 3 adenoid cystic carcinomas and 4 acinic cell carcinomas) and 3 normal submandibular glands using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) or western blotting. The mean expression levels of Bub1 mRNA and protein were higher in malignant tumors (0.12+/-0.028/1.75+/-0.53) than normal submandibular glands (0.042+/-0.014/0.19+/-0.044) and benign tumors (0.058+/-0.01/0.97+/-0.44). We found a significant association between the level of Bub1 mRNA/protein expression and clinical stage in malignant tumors (Mann-Whitney U test, p=0.019/p=0.016). We analyzed its relation with the proliferative activity monitored by the Ki-67 labeling index by immunohistochemistry as well as the expression of proliferating cell nuclear antigen (PCNA) by Western blotting. A significant correlation was found between Bub1 mRNA/protein expression and the Ki-67 labeling index in salivary gland tumors (Spearman's correlation coefficient by rank test, p=0.026/p=0.002). These results indicate that increased expression of the human Bub1 gene is closely linked to abnormal cell proliferation in malignant conditions.

• Authors: Hideo Shigeishi, Shingo Yoneda, Masayuki Taki, Takeshi Nobumori, Kouji Ohta, Koichiro Higashikawa, Wataru Yasui, Nobuyuki Kamata
• Journal: Oncology reports (Oncol Rep) Vol. 15 Issue 4 Pg. 933-8 (Apr 2006) ISSN: 1021-335X [Print] Greece
• PMID: 16525682 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ki-67 Antigen
• Proliferating Cell Nuclear Antigen
• RNA, Messenger
• Protein Kinases
• BUB1 protein, human
• Bub1 spindle checkpoint protein
• Protein Serine-Threonine Kinases

Topics

• Adenolymphoma (genetics, metabolism, pathology)
• Adenoma, Pleomorphic (genetics, metabolism, pathology)
• Blotting, Western
• Carcinoma, Acinar Cell (genetics, metabolism, pathology)
• Carcinoma, Adenoid Cystic (genetics, metabolism, pathology)
• Carcinoma, Mucoepidermoid (genetics, metabolism, pathology)
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Ki-67 Antigen (analysis)
• Neoplasm Staging
• Proliferating Cell Nuclear Antigen (analysis)
• Protein Kinases (analysis, genetics)
• Protein Serine-Threonine Kinases
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Salivary Gland Neoplasms (genetics, metabolism, pathology)