The proteasomal ATPase complex is required for stress-induced transcription in yeast.

Abstract	Sug1 and Sug2 are two of six ATPases in the 19S regulatory particle of the 26S proteasome. We have shown previously that these proteins play a non-proteolytic role in the transcription of the GAL genes in yeast. In this study, we probe the requirement for these factors in stress-induced transcription in yeast. It is known that proteasomal proteolysis is not required for these events. Indeed, proteasome inhibitors strongly stimulate expression of these stress response genes. However, shifting strains carrying temperature-sensitive alleles of SUG1 and SUG2 to the restrictive temperature strongly inhibited the expression of HSP26, HSP104 and GAD1 in response to heat shock or treatment with menadione bisulfate. Furthermore, chromatin immunoprecipitation analysis revealed the recruitment of Sug1, Sug2 and Cim5 (another of the ATPases), but not 20S proteasome core proteins, to the promoters of these genes. These data show that the non-proteolytic requirement for the proteasomal ATPases extends beyond the GAL genes in yeast and includes at least the heat and oxidative stress-responsive genes.
Authors	Rita Sulahian, Devanjan Sikder, Stephen Albert Johnston, Thomas Kodadek
Journal	Nucleic acids research (Nucleic Acids Res) Vol. 34 Issue 5 Pg. 1351-7 ( 2006) ISSN: 1362-4962 [Electronic] England
PMID	16517940 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References	HSP82 protein, S cerevisiae HSP90 Heat-Shock Proteins Heat-Shock Proteins Repressor Proteins Saccharomyces cerevisiae Proteins DNA Polymerase III Proteasome Endopeptidase Complex Adenosine Triphosphatases RPT4 protein, S cerevisiae RPT6 protein, S cerevisiae
Topics	Adenosine Triphosphatases (physiology) DNA Polymerase III (metabolism) Gene Expression Regulation, Fungal HSP90 Heat-Shock Proteins (genetics) Heat-Shock Proteins (biosynthesis, genetics) Heat-Shock Response Oxidative Stress Promoter Regions, Genetic Proteasome Endopeptidase Complex (metabolism) Repressor Proteins (physiology) Saccharomyces cerevisiae (enzymology, genetics) Saccharomyces cerevisiae Proteins (genetics, physiology) Transcription, Genetic

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