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Isolation, identification, and partial cDNA cloning of genomic RNA of jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis.

Abstract
The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis (jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and characterized. It has an insert of 2.1 kb which hybridizes to the same 8.7-kb RNA in all the JSRV-infected sheep lung washes tested but does not hybridize to maedi-visna virus, a sheep lentivirus often found coinfecting JSRV-infected lungs. Comparison of the amino acid sequence encoded by JS 46.1 with those encoded by other retroviruses revealed that JSRV has homology to the type D and B oncoviruses and to human endogenous retrovirus.
AuthorsD F York, R Vigne, D W Verwoerd, G Querat
JournalJournal of virology (J Virol) Vol. 65 Issue 9 Pg. 5061-7 (Sep 1991) ISSN: 0022-538X [Print] United States
PMID1651422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Gene Products, env
  • RNA, Viral
  • DNA
Topics
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Centrifugation, Isopycnic
  • Cloning, Molecular
  • DNA (genetics)
  • Gene Products, env (genetics)
  • Lentivirus (isolation & purification)
  • Lung (microbiology)
  • Molecular Sequence Data
  • Pulmonary Adenomatosis, Ovine (microbiology)
  • RNA, Viral (genetics, isolation & purification)
  • Retroviridae (genetics, isolation & purification)
  • Sheep

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