Abstract |
The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis ( jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and characterized. It has an insert of 2.1 kb which hybridizes to the same 8.7-kb RNA in all the JSRV-infected sheep lung washes tested but does not hybridize to maedi-visna virus, a sheep lentivirus often found coinfecting JSRV-infected lungs. Comparison of the amino acid sequence encoded by JS 46.1 with those encoded by other retroviruses revealed that JSRV has homology to the type D and B oncoviruses and to human endogenous retrovirus.
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Authors | D F York, R Vigne, D W Verwoerd, G Querat |
Journal | Journal of virology
(J Virol)
Vol. 65
Issue 9
Pg. 5061-7
(Sep 1991)
ISSN: 0022-538X [Print] United States |
PMID | 1651422
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gene Products, env
- RNA, Viral
- DNA
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Topics |
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Centrifugation, Isopycnic
- Cloning, Molecular
- DNA
(genetics)
- Gene Products, env
(genetics)
- Lentivirus
(isolation & purification)
- Lung
(microbiology)
- Molecular Sequence Data
- Pulmonary Adenomatosis, Ovine
(microbiology)
- RNA, Viral
(genetics, isolation & purification)
- Retroviridae
(genetics, isolation & purification)
- Sheep
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