Abstract | OBJECTIVE: METHODS: First, a mouse model of acute phase response was established by intraperitoneal injection of LPS. The relationship between hepcidin expression and dose or time of LPS injection was assessed. Then, electrophoretic mobility shift assay (EMSA) was performed to explore the possibility of the involvement of NF-kB in regulation of hepcidin gene transcription. Next, pAVU6+27- NF-kB, NF-kB p65-specific siRNA expression vector was constructed and transfected into mouse primary hepatocytes using DOTAP liposomal transfection reagents. Hepcidin expression changes after silencing of NF-kB p65 and hepcidin expression after LPS induction were tested. RESULTS:
Hepcidin expression showed a time and dose-dependent manner with regard to LPS injection. At 10 h after 50 microg LPS injection, hepcidin expression reached its peak. The result of EMSA exhibited an evident lag band at -53 - -64 bp, indicating regulation of hepcidin gene expression by NF-kB. After mouse primary hepatocytes were transiently transfected with NF-kB p65-specific siRNAs, Western blot showed that inhibition rate of NF-kB expression was 50%-67%. Hepcidin expression of transfected hepatocytes dropped down obviously in comparison with that of untransfected hepatocytes, and could not be induced by LPS. CONCLUSION:
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Authors | Rong-xia Liao, Jian-guo Sun, Xiao-lin Zhong, Jian-xin Zhou, Yuan-zhong Wang |
Journal | Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
(Zhonghua Gan Zang Bing Za Zhi)
Vol. 14
Issue 2
Pg. 118-23
(Feb 2006)
ISSN: 1007-3418 [Print] China |
PMID | 16494782
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- Antimicrobial Cationic Peptides
- Hamp protein, mouse
- Hepcidins
- Lipopolysaccharides
- NF-kappa B
- RNA, Small Interfering
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Topics |
- Acute-Phase Reaction
(chemically induced, genetics)
- Animals
- Anti-Bacterial Agents
- Antimicrobial Cationic Peptides
(genetics)
- Gene Expression Regulation
- Hepcidins
- Lipopolysaccharides
- Mice
- NF-kappa B
(genetics)
- RNA Interference
- RNA, Small Interfering
(genetics)
- Transcription, Genetic
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