Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions and chemical reagents.

Abstract

The efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10(6) TCID(50)/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56 degrees C for 60 min or longer reduced the infectivity of the virus from 2.6 x 10(7) to undetectable levels. Irradiation with ultraviolet light at 134 microW/cm(2) for 15 min reduced the infectivity from 3.8 x 10(7) to 180 TCID(50)/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID(50)/ml.

• Authors: Hiroaki Kariwa, Nobuhiro Fujii, Ikuo Takashima
• Journal: Dermatology (Basel, Switzerland) (Dermatology) Vol. 212 Suppl 1 Issue Suppl 1 Pg. 119-23 ( 2006) ISSN: 1018-8665 [Print] Switzerland
• PMID: 16490989 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents, Local
• Antiviral Agents
• Indicators and Reagents
• Acetone
• Formaldehyde
• Ethanol
• Povidone-Iodine
• Glutaral
• Methanol

Topics

• Acetone (pharmacology)
• Animals
• Anti-Infective Agents, Local (pharmacology)
• Antiviral Agents (pharmacology)
• Chlorocebus aethiops
• Ethanol (pharmacology)
• Formaldehyde (pharmacology)
• Glutaral (pharmacology)
• Hot Temperature
• Indicators and Reagents (pharmacology)
• Methanol (pharmacology)
• Povidone-Iodine (pharmacology)
• Severe acute respiratory syndrome-related coronavirus (drug effects, radiation effects)
• Ultraviolet Rays
• Vero Cells
• Virus Inactivation