Abstract | AIM: METHODS: Male Wistar rats (25) were divided into five groups. Those in group A received two injections of 3.2g/kg body weight L-Arg i.p. at an interval of 1h. In group MA, the rats were treated with 50 mg/kg body weight melatonin i.p. 30 min prior to L-Arg administration. In group AM, the rats received the same dose of melatonin 1h after L-Arg was given. In group M, a single dose of melatonin was administered as described previously. In group C the control animals received physiological saline injections i.p. All rats were exsanguinated 24 h after the second L-Arg injection. RESULTS: L-Arg administration caused severe necrotizing pancreatitis confirmed by the significant elevations in the serum amylase level, the pancreatic weight/body weight ratio (pw/bw), the pancreatic IL-6 content and the myeloperoxidase activity, relative to the control values. Elevation of the serum amylase level was significantly reduced in rats given melatonin following L-Arg compared to rats injected with L-Arg only. The activities of the pancreatic antioxidant enzymes ( Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and catalase (CAT)) were significantly increased 24 h after pancreatitis induction. Melatonin given in advance of L-Arg significantly reduced the pancreatic CAT activity relative to that in the rats treated with L-Arg alone. In the liver, L-Arg significantly increased the lipid peroxidation level, and the glutathione peroxidase and Cu/Zn-SOD activities, whereas the Mn-SOD activity was reduced as compared to the control rats. Melatonin pre-treatment prevented these changes. CONCLUSION:
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Authors | Annamaria Szabolcs, Russel J Reiter, Tamas Letoha, Peter Hegyi, Gabor Papai, Ilona Varga, Katalin Jarmay, Jozsef Kaszaki, Reka Sari, Zoltan Rakonczay Jr, Janos Lonovics, Tamas Takacs |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 12
Issue 2
Pg. 251-8
(Jan 14 2006)
ISSN: 1007-9327 [Print] United States |
PMID | 16482626
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Malondialdehyde
- Arginine
- Catalase
- Superoxide Dismutase
- Amylases
- Melatonin
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Topics |
- Acute Disease
- Amylases
(blood)
- Animals
- Arginine
(toxicity)
- Catalase
(metabolism)
- Lipid Peroxidation
(drug effects)
- Male
- Malondialdehyde
(analysis)
- Melatonin
(therapeutic use)
- Pancreatitis
(chemically induced, drug therapy)
- Rats
- Rats, Wistar
- Superoxide Dismutase
(metabolism)
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