B cells play an important role in lipopolysaccharide-induced bone resorption.

Abstract

The host immune system, especially activated T cells, plays a crucial role in inflammatory bone resorption and osteoclastogenesis. Previously, we showed that T cells are involved in inflammatory bone resorption in vivo. However, little is known about whether B cells are involved in inflammatory bone resorption and how B cells take part in osteoclastogenesis. Therefore, the aim of this study was to examine whether B c ells truly influence inflammatory bone resorption in vivo. Alveolar bone resorption in normal mice, in SCID mice that lack both B and T cells, and in B cell-reconstituted SCID mice was compared histopathologically after repeated injections of lipopolysaccharide (LPS) into the gingiva. Furthermore, we examined whether the B cells that are stimulated by LPS are involved in osteoclastogenesis in vitro. As a result, the B cell-reconstituted SCID mice showed stronger inflammatory bone resorption than the SCID mice. Also, in vitro, LPS-stimulated B cells enhanced osteoclastogenesis and anti-tumor necrosis factor (TNF)-alpha antibody completely blocked osteoclastogenesis induced by LPS-stimulated B cells. These results suggest that B cells promote inflammatory bone resorption through TNF-alpha.

• Authors: Y Kozuka, Y Ozaki, T Ukai, T Kaneko, Y Hara
• Journal: Calcified tissue international (Calcif Tissue Int) Vol. 78 Issue 3 Pg. 125-32 (Mar 2006) ISSN: 0171-967X [Print] United States
• PMID: 16467977 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Lipopolysaccharides
• Tumor Necrosis Factor-alpha

Topics

• Animals
• B-Lymphocytes (cytology, drug effects, metabolism)
• Bone Marrow Cells (cytology)
• Bone Resorption (chemically induced, immunology, pathology)
• Cells, Cultured
• Femur (cytology)
• Flow Cytometry
• Gingiva (drug effects, pathology)
• Immunohistochemistry
• Lipopolysaccharides (pharmacology)
• Lymphocyte Activation
• Male
• Mice
• Mice, Inbred Strains
• Mice, SCID
• Spleen (cytology)
• Tibia (cytology)
• Tumor Necrosis Factor-alpha (metabolism)