Abstract | PURPOSE: EXPERIMENTAL DESIGN: EphA2 mRNA and protein expression was investigated by reverse transcription-PCR and Western blot, respectively, in bladder cancer cell lines. In addition, the expression of EphA2, Ephrin A-1, and E-cadherin in tissues from patients with different stages of urinary bladder cancer was determined by immunohistochemistry. Furthermore, the ability of Ephrin A-1 to inhibit growth of bladder cancer cells was also investigated using an adenoviral delivery system. RESULTS: Western blot analysis showed high EphA2 expression in TCCSUP, T24, and UMUC-3 cell lines. In tissues, the staining intensity of EphA2 was less in normal urothelium but increased greatly in advancing stages of urothelial carcinoma (P < 0.05). Similarly, the staining intensity of Ephrin A-1 was low in normal tissues and high in cancerous tissues, but it was similar across the various stages of urothelial carcinoma (T(a)-T(4)). E-cadherin immunoreactivity decreased in urothelial cancer. Association of EphA2 and Ephrin A-1 expression was found to be significant between T(a) stage and T(1)-T(2) (P < 0.04) and T(a) and T(3)-T(4) stages (P < 0.0001). Adenovirus delivery of Ephrin A-1 inhibited proliferation of TCCSUP cells. CONCLUSION:
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Authors | Shaji Abraham, Deborah W Knapp, Liang Cheng, Paul W Snyder, Suresh K Mittal, Dinesh S Bangari, Michael Kinch, Lan Wu, Jay Dhariwal, Sulma I Mohammed |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 12
Issue 2
Pg. 353-60
(Jan 15 2006)
ISSN: 1078-0432 [Print] United States |
PMID | 16428472
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Cadherins
- Ephrin-A1
- RNA, Messenger
- Receptor, EphA2
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Topics |
- Adenoviridae
(genetics)
- Blotting, Western
- Cadherins
(genetics, metabolism)
- Cell Proliferation
- Ephrin-A1
(metabolism)
- Humans
- Immunoenzyme Techniques
- Lymphatic Metastasis
(pathology)
- Neoplasm Staging
- RNA, Messenger
(metabolism)
- Receptor, EphA2
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Tumor Cells, Cultured
- Urinary Bladder Neoplasms
(genetics, metabolism, pathology)
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