Abstract |
We investigated the effects of dietary administration of docosahexaenoic acid (DHA; C22:6n-3) on the levels of amyloid beta (A beta) peptide (1-40) and cholesterol in the nonionic detergent Triton 100 x-insoluble membrane fractions (DIFs) of the cerebral cortex and, also, on learning-related memory in an animal model of Alzheimer's disease (AD) rats infused with A beta peptide (1-40) into the cerebral ventricle. The infusion increased the levels of A beta peptide and cholesterol in the DIFs concurrently with a significant increase in reference memory errors (measured by eight-arm radial-maze tasks) compared with those of vehicle rats. Conversely, the dietary administration of DHA to AD-model rats decreased the levels of A beta peptide and cholesterol in the DIFs, with the decrease being more prominent in the DHA-administered rats. Regression analysis revealed a significant positive correlation between A beta peptide and each of cholesterol, palmitic acid and stearic acid, and between the number of reference memory errors and each of cholesterol, palmitic, stearic and oleic acid; moreover, a significant negative correlation was observed between the number of reference memory errors and the molar ratio of DHA to palmitic plus stearic acid. These results suggest that DHA-induced protection of memory deficits in AD-model rats is related to the interactions of cholesterol, palmitic acid or stearic acid with A beta peptides in DIFs where DHA ameliorates these interactions.
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Authors | Michio Hashimoto, Shahdat Hossain, Haqu Agdul, Osamu Shido |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1738
Issue 1-3
Pg. 91-8
(Dec 30 2005)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 16427803
(Publication Type: Journal Article)
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Chemical References |
- Amyloid beta-Peptides
- Detergents
- Fatty Acids
- Docosahexaenoic Acids
- Octoxynol
- Cholesterol
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Topics |
- Alzheimer Disease
(drug therapy)
- Amyloid beta-Peptides
(drug effects, metabolism, pharmacology)
- Animals
- Body Weight
(drug effects)
- Cell Membrane
(chemistry, drug effects, metabolism)
- Cerebral Cortex
(drug effects)
- Cholesterol
(metabolism)
- Detergents
(pharmacology)
- Disease Models, Animal
- Docosahexaenoic Acids
(pharmacology)
- Fatty Acids
(metabolism)
- Infusions, Intralesional
- Learning
(drug effects)
- Memory
(drug effects)
- Octoxynol
(pharmacology)
- Rats
- Rats, Wistar
- Regression Analysis
- Solubility
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