A pseudotype vesicular stomatitis virus containing Hantaan virus envelope glycoproteins G1 and G2 as an alternative to hantavirus vaccine in mice.

Abstract

We examined whether a vesicular stomatitis virus (VSV) pseudotype bearing the hantavirus envelope glycoproteins (GPs) G1 and G2 (VSVdeltaG*HTN) could be used as a safe and effective alternative to native hantavirus. Mice were immunized with purified particles of VSVdeltaG*HTN. After the second immunization, all mice produced anti-GP antibody as detected in ELISA and a neutralization test. After the third immunization, the mice were challenged with Hantaan virus. Neither anti-NP antibody production nor Hantaan virus-specific CD8 T-cell reactions were detected in these mice. The present study demonstrated the potential of using a pseudotype VSV system as a tool for developing a hantavirus vaccine.

• Authors: Byoung-Hee Lee, Kumiko Yoshimatsu, Koichi Araki, Megumi Okumura, Ichiro Nakamura, Jiro Arikawa
• Journal: Vaccine (Vaccine) Vol. 24 Issue 15 Pg. 2928-34 (Apr 05 2006) ISSN: 0264-410X [Print] Netherlands
• PMID: 16426712 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Vaccines, Synthetic
• Viral Envelope Proteins
• Viral Vaccines
• glycoprotein G1, Hantavirus
• glycoprotein G2, Hantavirus
• Interferon-gamma

Topics

• Animals
• Antibodies, Viral (blood)
• CD8-Positive T-Lymphocytes (immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Orthohantavirus (genetics, immunology)
• Hantavirus Infections (prevention & control)
• Interferon-gamma (biosynthesis)
• Mice
• Mice, Inbred BALB C
• Neutralization Tests
• Vaccines, Synthetic (genetics, immunology)
• Vesicular stomatitis Indiana virus (genetics, immunology)
• Viral Envelope Proteins (genetics, immunology)
• Viral Vaccines (genetics, immunology)