The effect of dehydroepiandrosterone sulfate (DHEAS) on myotonia: intracellular studies.

In order to find some appropriate medicine to suppress myotonia without decreasing muscle strength experiments were performed on myotonic (mto) mice whose Cl channel does not develop due to stop codon and serves as an animal model of myotonia. In myotonic dystrophy dehydroepiandrosterone is low in the serum and it has been reported that intravenous injections of DHEAS to human cases improves myotonia and activities of daily living.
Three pairs of heterozygote mto mice, SWR/J-Clcn1(adr-mto/+) and ten Wistar rats were used. We performed intracellular recordings of myotonia from mto mice and the drug effects on insertion myotonia were recorded from the hemidiaphragm preparations of mto mice with different concentrations of DHEAS. Isometric twitch tension was recorded from rat hemidiaphragm preparations in Tyrode's solution and the effect of DHEAS on the muscle twitch tension was measured at different concentrations of DHEAS from 100 mg/l to 300 mg/l. The effect of mexiletine on ITT was also measured.
In mto mice insertion myotonia was recorded as soon as the microelectrode was inserted in the muscle cells. When DHEAS was added to Tyrode's solution, insertion myotonia was suppressed. DHEAS decreased ITT up to 70% of the original value, though mexiletine decreased ITT to 30% of the original value. Therefore, the decrement of the muscle strength in DHEAS solution is much smaller than that of mexiletine.
Since myotonic dystrophy shows progressive muscle weakness in addition to myotonia, medications like DHEAS are more favorable than the typical Na channel blocker.
AuthorsHiroshi Nakazora, Teruyuki Kurihara
JournalInternal medicine (Tokyo, Japan) (Intern Med) Vol. 44 Issue 12 Pg. 1247-51 (Dec 2005) ISSN: 0918-2918 [Print] Japan
PMID16415544 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Dehydroepiandrosterone Sulfate
  • Action Potentials (drug effects, physiology)
  • Animals
  • Dehydroepiandrosterone Sulfate (therapeutic use)
  • Disease Models, Animal
  • Electrophysiology (methods)
  • Isometric Contraction (drug effects)
  • Mice
  • Muscle, Skeletal (drug effects, pathology, physiopathology)
  • Myotonia (drug therapy, metabolism, pathology)
  • Rats
  • Rats, Wistar

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