Mechanisms involved in the cardiovascular responses to opioid products of proenkephalin in the anaesthetised rat.

Abstract

1. Cardiovascular effects of opioid peptide products of proenkephalin, [Met] enkephalin (ME), [Leu] enkephalin (LE), [Met] enkephalyl Arg6-Phe7 (MEAP) and [Met] enkephalyl Arg6-Gly7-Leu8 (MEAGL) have been studied in urethane-anaesthetised rats. 2. ME, LE, MEAP and MEAGL produced vasodepression and bradycardia mediated by mu-opioid receptors. 3. Atypical responses to MEAP were observed in a quarter of the animals studied showing tachycardia and pressor effects. This response was probably due to the release of the dipeptide Arg-Phe which exerted its effects at sympathetic ganglia. 4. Studies with the peptidase inhibitors captopril and bestatin showed a differential potentiation of the cardiovascular effects of the proenkephalin products by inhibition of angiotensin converting enzyme and aminopeptidase. 5. The effects of vagotomy, pithing and studies with atropine, and N-methyl levallorphan were used to demonstrate that, for all four proenkephalin peptides, cardiovascular effects were mediated by peripheral opioid receptors and transmission to the CNS via vagal afferents.

• Authors: H Douglas, I Kitchen
• Journal: General pharmacology (Gen Pharmacol) Vol. 23 Issue 2 Pg. 269-77 (Mar 1992) ISSN: 0306-3623 [Print] England
• PMID: 1639241 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endorphins
• Enkephalins
• Narcotics
• Protein Precursors
• proenkephalin

Topics

• Anesthesia
• Animals
• Blood Gas Analysis
• Blood Pressure (drug effects)
• Decerebrate State
• Endorphins (pharmacology)
• Enkephalins (pharmacology)
• Heart Rate (drug effects)
• Hemodynamics (drug effects)
• Male
• Narcotics (pharmacology)
• Protein Precursors (pharmacology)
• Rats
• Rats, Inbred Strains
• Vagotomy