Sdfetal rat myocardial cells were isolated and cultured in vitro. The released amounts of
creatine kinase (CK) and
lactate dehydrogenase (LDH) from the myocardial cells in the culture supernatant at 6 hour after
hypoxia and after
glycine treatment were determined with ultraviolet spectrophotometer. The expression of the alpha1 subunits of
glycine receptor (GlyRalpha1) in the myocardial cells was detected by immunofluorescent histochemistry. The changes in the intracellular
calcium content and the membrane potential of the myocardial cells were determined by
laser confocal microscopy.
RESULTS: The release of CK and LDH in the culture supernatant increased significantly at 6 h after
hypoxia [(393.8 +/- 5.3), (1564 +/- 41) U/L] compared with those before
hypoxia, while their levels were obviously decreased after
glycine treatment [(56.3 +/- 2.7), (716 +/- 18) U/L, (P <0.01)] compared with those before
glycine treatment. There was positive expression of GlyRalpha1 in myocardial cells before and after
hypoxia. The average fluorescent intensity of intracellular
calcium at 6 hours after
hypoxia (139 +/- 29) was significantly higher than that before
hypoxia (27 +/- 8, P < 0.01), while it was obviously lower (51 +/- 11) after
glycine treatment compared with that at 6 hours after
hypoxia,but it was evidently higher than that before
hypoxia (P <0.01). The membrane potential 6 hours after
hypoxia (62 +/- 9) was obviously lower than that before
hypoxia (177 +/- 20, P < 0.01), but it was obviously higher after
glycine treatment (123 +/- 16) than that at 6 hours after
hypoxia (P < 0.01).
CONCLUSION: