Abstract | BACKGROUND: The pathophysiology of the testicular damage in varicocele has not been completely understood. Oxidative stress and related sperm DNA damage have been identified as significant causes of male infertility. The current study was designed to determine the extent of sperm nuclear DNA damage in patients with varicocele and to examine its relationship with oxidative stress. METHODS: RESULTS: DNA fragmentation index (DFI) (percentage of sperm with denatured DNA) values and the percentage of TUNEL-positive cells were significantly greater in patients with varicocele, either with normal (DFI, 20.7 +/- 4.0; TUNEL positive, 26.1 +/- 3.2) or with abnormal (DFI, 35.5 +/- 9.0; TUNEL positive, 32.2 +/- 4.1) semen profile, compared with controls (DFI, 7.1 +/- 0.9; TUNEL positive, 14.2 +/- 1.2). Similarly, ROS levels were significantly higher (P < 0.01) in both groups of patients with varicocele. CONCLUSIONS: The presence of a varicocele is associated with high levels of DNA-damage spermatozoa even in the presence of normal semen profile. The results also indicate that oxidative damage is associated with sperm DNA damage in these patients.
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Authors | R Smith, H Kaune, D Parodi, M Madariaga, R Rios, I Morales, A Castro |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 21
Issue 4
Pg. 986-93
(Apr 2006)
ISSN: 0268-1161 [Print] England |
PMID | 16361286
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Reactive Oxygen Species
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Topics |
- Biomarkers
- DNA Damage
- DNA Fragmentation
- Humans
- In Situ Nick-End Labeling
- Male
- Oxidative Stress
(physiology)
- Reactive Oxygen Species
(metabolism)
- Spermatozoa
(ultrastructure)
- Varicocele
(genetics)
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