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Immunohistochemical and genetic analysis of Chinese nasal natural killer/T-cell lymphomas.

Abstract
Nasal natural killer/T-cell lymphoma (N-NK/T-L) is prevalent in China. To further characterize this neoplasm, 36 cases of N-NK/T-L from 304 cases of malignant lymphomas in the north China area were investigated by histopathology, immunophenotyping, and genomic analysis of c-kit, in comparison with 11 cases of B-cell lymphoma (BCL) at the same region and 5 cases of nodal peripheral T-cell lymphoma (PTCL) (unspecified). Histopathologically, N-NK/T-L was characterized by coagulative necrosis, inflammatory background, and angiodestructive growth pattern. In 36 cases of N-NK/T-L, 27 cases (75.0%) were stained for CD45RO and 25 (72.2%) for CD3epsilon. Thirty cases (83.3%) were positive for T-cell intracellular antigen-1, 22 (61.1%) for granzyme B, 18 (50.0%) for CD56, and 11 (30.6%) for CD30, whereas none was positive for CD117. All 5 cases of PTCL displayed positive staining for CD45RO and T-cell intracellular antigen-1, 3 cases for CD3epsilon, but only 1 case for granzyme B. All 11 BCLs presented positive staining for CD20 and CD79a but negative for other antibodies. A significant relationship was observed between neoplastic cells pleomorphism and granzyme B expression (P < .05). Despite the fact that all cases were negative for CD117 staining, genomic sequences of c-kit 11 and exon 17 sequencing showed that 8 (26%) of 31 cases N-NK/T-L proved to contain genomic mutations, including 4 cases in exon 11 and 4 in exon 17. For the control group, only 1 (9%) of 11 BCLs and 1 (20%) of 5 cases of PTCL were detected to harbor mutations in exon 11. All mutations detected in 3 groups were missense by base substitution, and codes 571, 572, and 821 were hot spots. The results suggested that, in addition to histological features and routine immunophenotyping, granzyme B expression should be a more reliable marker in correct diagnosis of N-NK/T-L, and genetic analysis of c-kit mutation should be helpful in the diagnosis of this tumor.
AuthorsTing Li, Bo Zhang, Yuhong Ye, Hongfang Yin
JournalHuman pathology (Hum Pathol) Vol. 37 Issue 1 Pg. 54-60 (Jan 2006) ISSN: 0046-8177 [Print] United States
PMID16360416 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • DNA, Neoplasm
  • Proto-Oncogene Proteins c-kit
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor (metabolism)
  • China
  • DNA Mutational Analysis
  • DNA, Neoplasm (analysis)
  • Female
  • Humans
  • Immunohistochemistry
  • Immunophenotyping
  • Killer Cells, Natural (metabolism, pathology)
  • Lymphoma, B-Cell (genetics, metabolism, pathology)
  • Lymphoma, T-Cell, Peripheral (genetics, metabolism, pathology)
  • Male
  • Middle Aged
  • Mutation
  • Nose Neoplasms (genetics, metabolism, pathology)
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-kit (genetics, metabolism)

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