[Standardization of an in-house ELISA for pertussis serology and its application in a seroepidemiological study].
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| Abstract |
Seroepidemiological studies need sensitive, practicle and cost-effective methods. For pertussis serosurveillance, an in-house ELISA for antipertussis-toxin (PT) and anti-filamentous-hemagglutinin (FHA) were established in our laboratory and compared by a Ball-ELISA which had been reported to be reliable previously. Sixty sera with various antibody titers were tested by both of the methods. The correlation coefficients between two methods were 0.729 and 0.776 for anti-PT and anti-FHA, respectively, and regression coefficients were 0.623 and 0.693, respectively. The in-house ELISA was applied to a serosurvey including 373 healthy subjects (6 months-91 years old) in Turkey to observe the results. The moving averages of both antibodies were increased until 10 years old, reaching to 31 EU/ml for anti-PT and 65 EU/ml for anti-FHA and kept around this level in the older ages. The in-house ELISA was found to be reliable and the serosurvey results obtained by ELISA showed a characteristic distribution of antibody titers in each age group.
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| Authors | Nilay Cöplü, Berrin Esen, Demet Kurtoğlu, Aysegül Gözalan, Kikuko Miyamura, Iwao Yoshida |
| Journal | Mikrobiyoloji bulteni (Mikrobiyol Bul) Vol. 39 Issue 3 Pg. 281-9 (Jul 2005) ISSN: 0374-9096 [Print] Turkey |
| Vernacular Title | Laboratuvarimizda hazirlanan ELISA testinin boğmaca serolojisinde standardizasyonu ve seroepidemiyolojik bir calişmada kullanilmasi. |
| PMID | 16358487 (Publication Type: Journal Article, Validation Study) |
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