HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio.

Abstract
Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and compared with the ALAD 1-1 genotype group. While no significant differences were found in Pb-B, subjects from the ALAD 1-2/2-2 genotype group presented significantly higher Pb-P concentrations and %Pb-P/Pb-B ratios (0.89+/-0.07 microg/l, and 1.45+/-0.10%, respectively) when compared with subjects from the ALAD 1-1 genotype group (0.44+/-0.05 microg/l, and 0.48+/-0.02, respectively; both P<0.0001). The higher %Pb-P/Pb-B ratios in carriers of the ALAD-2 allele compared with noncarriers indicate that ALAD 1-2/2-2 subjects are probably at increased health risks associated with lead exposure.
AuthorsMarcelo F Montenegro, Fernando Barbosa Jr, Valeria C Sandrim, Raquel F Gerlach, Jose E Tanus-Santos
JournalArchives of toxicology (Arch Toxicol) Vol. 80 Issue 7 Pg. 394-8 (Jul 2006) ISSN: 0340-5761 [Print] Germany
PMID16341691 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Lead
  • Porphobilinogen Synthase
Topics
  • Adolescent
  • Adult
  • Brazil
  • Environmental Exposure (analysis)
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Lead (blood)
  • Lead Poisoning (genetics, metabolism)
  • Male
  • Middle Aged
  • Polymorphism, Restriction Fragment Length
  • Porphobilinogen Synthase (blood, genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: