Abstract | PURPOSE: Nasal natural killer (NK)/ T-cell lymphoma is associated with EBV and has distinct clinical and histologic features. However, little is known about its genetic features. In this study, we examined the genes expressed by SNK-6 and SNT-8 cells, which were established from nasal NK/ T-cell lymphomas, and found that interleukin (IL)-9 was specifically expressed in these two cell lines. EXPERIMENTAL DESIGN: RESULTS: In cDNA array, expression of IL-9 mRNA was much higher in SNK-6 and SNT-8 cells than in NK-92 cells from non-nasal NK-cell lymphoma and peripheral blood mononuclear cells from healthy volunteers. Furthermore, IL-9 was specifically expressed by SNK-6 and SNT-8 cells but not by other NK-cell, NK-like T-cell, and T-cell lymphoma/ leukemia cell lines. IL-9 receptor was also expressed on the surfaces of SNK-6 and SNT-8 cells. An IL-9-neutralizing antibody inhibited the growth of these two cell lines, whereas recombinant human IL-9 enhanced their growth. Most significantly, IL-9 was present in biopsies and sera from patients with this lymphoma. CONCLUSIONS: These results suggest that IL-9 plays an important role in nasal NK/ T-cell lymphoma possibly via an autocrine mechanism.
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Authors | Toshihiro Nagato, Hiroya Kobayashi, Kan Kishibe, Miki Takahara, Takeshi Ogino, Hideyuki Ishii, Kensuke Oikawa, Naoko Aoki, Keisuke Sato, Shoji Kimura, Norio Shimizu, Masatoshi Tateno, Yasuaki Harabuchi |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 23
Pg. 8250-7
(Dec 01 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 16322282
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- IL9R protein, human
- Interleukin-9
- RNA, Messenger
- Receptors, Interleukin
- Receptors, Interleukin-9
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Topics |
- Autocrine Communication
- Biopsy
- Case-Control Studies
- Enzyme-Linked Immunosorbent Assay
- Epstein-Barr Virus Infections
(genetics, pathology)
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
(physiology)
- Herpesvirus 4, Human
(genetics)
- Humans
- Interleukin-9
(genetics)
- Killer Cells, Natural
(pathology)
- Lymphoma, T-Cell
(genetics, pathology, virology)
- Nose Neoplasms
(genetics, pathology, virology)
- Oligonucleotide Array Sequence Analysis
- RNA, Messenger
(metabolism)
- Receptors, Interleukin
(genetics)
- Receptors, Interleukin-9
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Cells, Cultured
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