Abstract | BACKGROUND:
HBOC-201 is an ultra purified bovine hemoglobin solution. It has already been used in clinical phase II/III trials for emergency treatments. Animal experiments have shown that HBOC-201 is highly effective in tissue oxygenation. The study was performed in order to assess the potential of low dose HBOC-201 to improve tumor oxygenation. METHODS: 30 rats with a subcutaneously growing rhabdomyosarcoma R1H tumor were randomly assigned either to be ventilated with carbogen (n = 10), or to receive an IV injection of 0.3 g/kg HBOC-201 (n = 10) or a combination of 0.3 g/kg HBOC-201 and carbogen breathing (n = 10). Under general anesthesia the effects of the respective treatment on the tissue oxygen tension (tpO2) of the tumor were determined using a flexible stationary probe at baseline (b) and 15 and 60 min after application of the respective medication. RESULTS:
HBOC-201 alone failed to improve tumor tpO2 (b: 1.3 +/- 1.2mmHg; 15min: 1.4 +/- 1 mmHg; 60min: 1 +/- 1 mmHg). In combination with carbogen the mean tpO2 of the tumor raised in comparison to baseline values (b: 3.1 +/- 4.6 mmHg; mmHg; 15min: 8.5 +/- 11*mmHg; 60min: 4.8 +/- 5mmHg; *p < 0.05 vs. b), but this effect was less pronounced than the increase in tpO2 by carbogen alone (b: 3.4 +/- 3.4mmHg; 15min: 9 +/- 10* mmHg; 60 min: 13 +/- 19* mmHg; *p < 0.05 vs. b). CONCLUSION:
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Authors | André Gottschalk, Annette Raabe, Matthias Hommel, Christian Rempf, Marc Freitag, Thomas Standl |
Journal | Artificial cells, blood substitutes, and immobilization biotechnology
(Artif Cells Blood Substit Immobil Biotechnol)
Vol. 33
Issue 4
Pg. 379-89
( 2005)
ISSN: 1073-1199 [Print] England |
PMID | 16317957
(Publication Type: Journal Article)
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Chemical References |
- Blood Substitutes
- Hemoglobins
- Carbon Dioxide
- HBOC 201
- carbogen
- Oxygen
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Topics |
- Animals
- Blood Substitutes
(administration & dosage, pharmacology)
- Carbon Dioxide
(administration & dosage, pharmacology)
- Drug Evaluation, Preclinical
- Hemoglobins
(administration & dosage, pharmacology)
- Hypoxia
(drug therapy)
- Oxygen
(administration & dosage, metabolism, pharmacology)
- Rats
- Rhabdomyosarcoma
(metabolism, pathology)
- Tissue Distribution
(drug effects)
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