[Rats resistance to fecal peritonitis when subjected to total splenectomy and auto-implant of the spleen in the retroperitoneum].

Abstract

PURPOSE:

This study was developed to evaluated the effects of spleen transplants in the protection against abdominal sepsis in young and old Wistar rats.

METHODS:

Fifty-four young and old Wistar rats were utilized (90 and 180 days respectively) being distributed in three groups as follows: Sham Group, where the animal were submitted to laparotomy with manipulation of only intestine (n=12 young and adult animals), Splenectomy Group, in which the animals were subjected to total splenectomy (n=20 young and adult animals) and an Auto-implant Group, where the animals were subjected to total splenectomy and a third of the spleen was implanted in a bag in the retroperitoneum (n=22 young and adult animals). After three months the animals were subjected to a new laparotomy with binding and caecum perforation to stimulate abdominal sepsis and were accompanied until the moment of death to establish a mortality curve.

RESULTS:

There was recuperation of the spleen implanted in all animals. There was no significant difference in the time of death among the groups. The young animals apparently had a better response, however it was not statistically significant.

CONCLUSION:

This study demonstrated that spleen implant in the retroperitoneum do not prove to be an effective protection against abdominal sepsis in comparison with the splenectomized rats.

• Authors: Darlan de Medeiros Kestering, Armando José d'Acampora, Débora Cadore Farias, Sandro Polidoro Berni Brum, Jorge Bins Ely
• Journal: Acta cirurgica brasileira (Acta Cir Bras) 2005 Nov-Dec Vol. 20 Issue 6 Pg. 473-7 ISSN: 0102-8650 [Print] Brazil
• Vernacular Title: Resistência de ratos à peritonite fecal quando submetidos a esplenectomia e auto-implante do baço no retroperitônio.
• PMID: 16302085 (Publication Type: Comparative Study, English Abstract, Journal Article)

Topics

• Age Factors
• Animals
• Feces (microbiology)
• Peritonitis (prevention & control)
• Rats
• Rats, Wistar
• Retroperitoneal Space (surgery)
• Spleen (transplantation)
• Splenectomy
• Time Factors
• Transplantation, Autologous