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Type I interferons protect mice against enterovirus 71 infection.

Abstract
In this study, the contribution of type I interferons (IFNs) to protection against infection with enterovirus 71 (EV71) was investigated using a murine model where the virus was administrated to neonatal Institute of Cancer Research (ICR) mice by either the intraperitoneal (i.p.) or the oral route. In i.p. inoculated mice, post-infection treatment of dexamethasone (5 mg kg(-1) at 2 or 3 days after infection) exacerbated clinical symptoms and increased the tissue viral titre. In contrast, polyriboinosinic : polyribocytidylic acid [poly(I : C); 10 or 100 microg per mouse at 12 h before infection], a potent IFN inducer, improved the survival rate and decreased the tissue viral titres after EV71 challenge, which correlated with an increase in serum IFN-alpha concentration, the percentage of dendritic cells, their expression of major histocompatibility complex class II molecule and IFN-alpha in spleen. Treatment with a neutralizing antibody for type I IFNs (10(4) neutralizing units per mouse, 6 h before and 12 h after infection) resulted in frequent deaths and higher tissue viral load in infected mice compared with control mice. In contrast, an early administration of recombinant mouse IFN-alphaA (10(4) U per mouse for 3 days starting at 0, 1 or 3 days after infection) protected the mice against EV71 infection. In vitro analysis of virus-induced death in three human cell lines showed that human type I IFNs exerted a direct protective effect on EV71. It was concluded that type I IFNs play an important role in controlling EV71 infection and replication.
AuthorsMing-Liang Liu, Yi-Ping Lee, Ya-Fang Wang, Huan-Yao Lei, Ching-Chuan Liu, Shih-Min Wang, Ih-Jen Su, Jen-Reng Wang, Trai-Ming Yeh, Shun-Hua Chen, Chun-Keung Yu
JournalThe Journal of general virology (J Gen Virol) Vol. 86 Issue Pt 12 Pg. 3263-3269 (Dec 2005) ISSN: 0022-1317 [Print] England
PMID16298971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies
  • Antiviral Agents
  • CD11c Antigen
  • CD8 Antigens
  • Histocompatibility Antigens Class II
  • Interferon Type I
  • Polynucleotides
  • Dexamethasone
  • Leukocyte Common Antigens
  • Poly I-C
Topics
  • Animals
  • Antibodies (immunology)
  • Antiviral Agents (pharmacology)
  • CD11c Antigen (analysis)
  • CD8 Antigens (analysis)
  • Cell Line
  • Cytopathogenic Effect, Viral (drug effects)
  • Dendritic Cells
  • Dexamethasone (pharmacology)
  • Disease Models, Animal
  • Enterovirus (drug effects)
  • Enterovirus Infections (drug therapy, prevention & control)
  • Histocompatibility Antigens Class II (analysis)
  • Humans
  • Interferon Type I (immunology, pharmacology)
  • Leukocyte Common Antigens (analysis)
  • Mice
  • Mice, Inbred ICR
  • Poly I-C (pharmacology)
  • Polynucleotides (pharmacology)
  • Spleen (immunology)

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