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The immunoreactivity and activity of adenylate cyclase type I are changed in the hippocampal CA1 region after transient forebrain ischemia in gerbils.

Abstract
Adenylate cyclase (AC) has a specific sensitivity to Ca2+/calmodulin. AC-I, one of the mediator of learning and memory, plays an important role in signal transduction underlying learning and memory function. In the present study, we found ischemia-related changes of AC-I in the hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia in gerbils. In the sham-operated group, AC-I immunoreactive neurons were detected in pyramidal and non-pyramidal cells in the hippocampus proper. AC-I immunoreactivity was significantly increased at 3 h in the CA1 region after ischemic insult. Thereafter, AC-I immunoreactivity was gradually decreased. Four days after ischemic insult, AC-I-immunoreactive CA1 pyramidal cells in the stratum pyramidale were very few due to delayed neuronal death. The results of Western blot analysis showed that changes of AC-I protein contents were similar to immunohistochemical data after ischemic insult. Gpp(NH)p-dependent AC-I activity in hippocampal CA1 region was not changed in all groups, while Ca2+/calmodulin-dependent AC-I activity in hippocampal CA1 region was significantly decreased 24 h after ischemia-reperfusion. These results suggest that the decrease of AC-I activity may be associated with impairment of neurodevelopment and neuroplasticity including learning and memory although the AC-I immunoreactivity was maintained 24 h postischemic group compared to that of the sham-operated group.
AuthorsIn Koo Hwang, Ki-Yeon Yoo, Young-Sil Kim, Boo Kyoung Jung, Hua Li, Do-Hoon Kim, Tae-Cheon Kang, Hyung-Cheul Shin, Moo Ho Won
JournalJournal of the neurological sciences (J Neurol Sci) Vol. 240 Issue 1-2 Pg. 93-8 (Jan 15 2006) ISSN: 0022-510X [Print] Netherlands
PMID16297936 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adenylyl Cyclases
Topics
  • Adenylyl Cyclases (metabolism)
  • Analysis of Variance
  • Animals
  • Blotting, Western (methods)
  • Disease Models, Animal
  • Gerbillinae
  • Hippocampus (enzymology, pathology)
  • Immunohistochemistry (methods)
  • Ischemic Attack, Transient (metabolism, pathology)
  • Male
  • Neurons (metabolism)
  • Reperfusion
  • Staining and Labeling (methods)
  • Time Factors

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