[Effect of heat shock protein 70 on nuclear factor-KappaB/inhibitor protein-alpha of nuclear factor-KappaB in brain edema induced by infection in rat].

Abstract

OBJECTIVE:

To study the effect of heat shock protein 70 (HSP70) on activation of nuclear factor-KappaB (NF-KappaB) and degradation of inhibitor KappaB-alpha protein (I-Kappa B alpha) in brain edema induced by Pertussis bacilli infection in rats.

METHODS:

Brain edema was induced by injection of Pertussis bacilli suspension via the left internal carotid artery. Seventy-two Sprague-Dawley rats were divided randomly into brain edema group, pretreatment with heat shock group, and normal saline control group. The rats were sacrificed 4, 8 and 24 hours after injection of Pertussis bacilli and normal saline, respectively. HSP70 expression and the degradation of I-Kappa B alpha were assayed in all animals with Western blot analysis. Electrophoretic mobility shift assay (EMSA) was performed to assess NF-KappaB activation of nuclear extract of neurones.

RESULTS:

The results showed that HSP70 expression was significantly increased in heat shock group compared with control group and brain edema group. The activity of NF-KappaB started to increase at 2 hours in brain edema group and peaked at 24 hours. The expression of I-Kappa B alpha began to decrease at 2 hours and reached the lowest level at 24 hours. In heat shock group the activity of NF-KappaB complexes was lower than that in brain edema group, and the expression of I-Kappa B alpha higher than that in brain edema group at corresponding time points.

CONCLUSION:

HSP70 shows a protective effect on development of brain edema as its expression increases. The mechanism might be associated with its inhibitory effect on degradation of I-Kappa B alpha and activation of NF-KappaB.

• Authors: Ding-an Mao, Yu-Jia Yang, Yan Yu
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 11 Pg. 644-6 (Nov 2005) ISSN: 1003-0603 [Print] China
• PMID: 16297314 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP70 Heat-Shock Proteins
• I-kappa B Proteins
• NF-kappa B

Topics

• Animals
• Brain (metabolism)
• Brain Edema (metabolism)
• Disease Models, Animal
• Female
• HSP70 Heat-Shock Proteins (metabolism)
• I-kappa B Proteins (metabolism)
• Male
• NF-kappa B (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Whooping Cough (metabolism, pathology)