The dental pulp: inflammatory markers and vital bleaching.

Abstract

PURPOSE:

To evaluate the expression of specific neuropeptides associated with inflammation, substance-P (SP) and calcitonin gene-related peptide (CGRP), after night guard vital bleaching with 10% carbamide peroxide gel.

METHODS:

10 patients with four caries free premolars scheduled for orthodontic extraction had these teeth bleached with 10% Opalescence as follows: tooth #28, no treatment (control); #5, 4 days of bleaching; #12, 2 weeks of bleaching; #21, 2 weeks of bleaching, followed by 2 weeks without treatment. All teeth in the four groups were extracted at the same time. Immediately after extraction the teeth were frozen at -197 degrees C in liquid nitrogen. The pulp samples were prepared for enzyme-linked immunoabsorbent assay (ELISA) to determine the concentrations of the SP, CGRP and total protein in the pulp tissue.

RESULTS:

The analysis of mean SP and mean CGRP/ng protein/ml resulted in no significant differences among the four groups. It was concluded that the study did not demonstrate an increase in the release of SP and/or CGRP during night guard vital bleaching of teeth with 10% Opalescence.

• Authors: Orlando J Fugaro, Jessica O Fugaro, Bruce Matis, Richard L Gregory, Michael A Cochran, Ivar Mjör
• Journal: American journal of dentistry (Am J Dent) Vol. 18 Issue 4 Pg. 229-32 (Aug 2005) ISSN: 0894-8275 [Print] United States
• PMID: 16296427 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Drug Combinations
• Oxidants
• Peroxides
• Carbamide Peroxide
• Substance P
• Urea
• Calcitonin Gene-Related Peptide

Topics

• Adolescent
• Adult
• Biomarkers
• Calcitonin Gene-Related Peptide (analysis, biosynthesis)
• Carbamide Peroxide
• Child
• Dental Pulp (drug effects, metabolism)
• Drug Combinations
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Male
• Neurogenic Inflammation (chemically induced)
• Oxidants (toxicity)
• Peroxides (toxicity)
• Substance P (analysis, biosynthesis)
• Tooth Bleaching (adverse effects)
• Urea (analogs & derivatives, toxicity)