Preliminary study on mouse interleukin-21 application in tumor gene therapy.

Abstract

Interleukin-21 (IL-21) is a recently characterized T cell-derived cytokine with a significant homology to IL-2, IL-4 and IL-15. To determine whether IL-21 has broad immunoregulatory activity and can stimulate durable antitumour responses, we constructed mouse IL-21 (mIL-21) recombinant plasmid and evaluated its antitumor efficacy. Mouse IL-21 cDNA was amplified from Con A-activated mouse T cells by RT-PCR. Recombinant pcDNA3.1/mIL-21 was constructed and transfected into Sp2/0 cells. Mouse IL-21 expression was analyzed by Western blotting and its activities were detected by 3H-TdR incorporation and MTT assay. The recombinant pcDNA3.1/mIL-21 was injected s.c. into tumor lump. Tumor size, weight, the activities of CTLs, NK cells and LAK cells and serum IFN-gamma level were measured for evaluating mIL-21 mediated antitumor responses. The results indicated that mIL-21 was correctly expressed in Sp2/0 cells and it can improve the proliferation of T cells and B cells, and enhance NK cytotoxic activity in vitro. The activities of CTL and NK cells, and serum IFN-gamma level were significantly improved, furthermore the tumor growth was obviously suppressed in pcDNA3.1/mIL-21 treated mice. However, the LAK activity did not alter significantly. Taken together, this study suggests that the injection with recombinant plasmid containing mIL-21 is a potential approach for tumor gene therapy.

• Authors: Jun Dou, Guobin Chen, Jing Wang, Fengshu Zhao, Junsong Chen, Xuesong Fang, Quan Tang, Lili Chu
• Journal: Cellular & molecular immunology (Cell Mol Immunol) Vol. 1 Issue 6 Pg. 461-6 (Dec 2004) ISSN: 1672-7681 [Print] China
• PMID: 16293216 (Publication Type: Journal Article)

Chemical References

• Interleukins
• Recombinant Proteins
• Interferon-gamma
• interleukin-21

Topics

• Animals
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic
• Genetic Therapy (methods)
• Genetic Vectors (genetics)
• Injections
• Interferon-gamma (metabolism)
• Interleukins (genetics, immunology, metabolism)
• Killer Cells, Natural (immunology)
• Mice
• Mice, Inbred BALB C
• Neoplasms (genetics, immunology, pathology, therapy)
• Recombinant Proteins (genetics, metabolism)
• T-Lymphocytes, Cytotoxic (immunology)
• Xenograft Model Antitumor Assays