The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells.
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| Abstract |
L-plastin, a gene that codes for an actin-bundling protein, is upregulated in the metastatic colon cancer cell line SW620, when compared to its premetastatic counterpart SW480. The aim of our study was to characterise the effect of L-plastin overexpression on SW480 cells in the context of the acquisition of a metastatic phenotype. SW480 cell lines overexpressing L-plastin were established (SW480-LPL). Analysis of these cell lines revealed significantly higher rates of proliferation and invasion than the control cell line (SW480-Ctrl). In addition, the expression of E-cadherin was lost from SW480-LPL cells. Treatment of SW480-LPL cells with cytochalasin B, an inhibitor of endocytosis, attenuated the loss of E-cadherin expression in these cells. The association of L-plastin overexpression with an increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis (supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html).
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| Authors | Eilis Foran, Peter McWilliam, Dermot Kelleher, David T Croke, Aideen Long |
| Journal | International journal of cancer (Int J Cancer) Vol. 118 Issue 8 Pg. 2098-104 (Apr 15 2006) ISSN: 0020-7136 [Print] United States |
| PMID | 16287074 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright (c) 2005 Wiley-Liss, Inc. |
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