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[Ultrastructural immunochemical study of pathogenic Burkholderia capsule].

Abstract
The capsular structures of Burkholderia pseudomallei, B. mallei, B. cepacia and their avirulent noncapsular mutants were studied with the use of electron ahd immunocytochemical techniques. For this purpose, antimelio-idosis monoclonal antibodies (McAb) G11 and 1 G2, epitope-aimed at capsular glycopyotein of 200 kD and outer-membrane proteins of 42 and 39 kD, were used. As revealed in this study, the typical causative agents of melioidosis and glanders formed the capsule and exhibited high virulence due to the antiphagocytic activity of 200 kD glycoprotein, whose epitopes were found to be incorporated into the capsule, in contrast to avirulent variants and B. cepacia, found to have no such structure. The recognition of the membrane determinants of McAb 1 G2 on the outer-membrane surface of the non-capsular variants of microbes known to be the causative agents of melioidosis and glanders was indicative of absence of the capsule in these microbial cells. These data concerning the role of 200 kD antigen in virulence, its structural and functional characteristics may be efffectively used in the study of the pathogenetic mechanisms of melioidosis and glanders, as well as in the construction of preparations for their immunodiagnostics and prophylaxis.
AuthorsN N Piven', V V Alekseev, S F Popov, N P Khrapova, E V Prokhvatilova, D V Viktorov, N G Plekhanova, V I Kapliev, I V Avrorova
JournalZhurnal mikrobiologii, epidemiologii, i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) 2005 Sep-Oct Issue 5 Pg. 19-24 ISSN: 0372-9311 [Print] Russia (Federation)
PMID16279528 (Publication Type: Comparative Study, English Abstract, Journal Article)
Chemical References
  • Bacterial Proteins
  • Epitopes
  • Membrane Glycoproteins
  • Arabinose
Topics
  • Arabinose (deficiency, genetics)
  • Bacterial Capsules (chemistry, ultrastructure)
  • Bacterial Proteins (chemistry)
  • Blotting, Western
  • Burkholderia (genetics, pathogenicity, ultrastructure)
  • Burkholderia cepacia (genetics, pathogenicity, ultrastructure)
  • Burkholderia mallei (genetics, pathogenicity, ultrastructure)
  • Burkholderia pseudomallei (genetics, pathogenicity, ultrastructure)
  • Epitopes (chemistry)
  • Immunohistochemistry
  • Membrane Glycoproteins (chemistry)
  • Microscopy, Electron
  • Molecular Weight
  • Virulence (genetics)

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