Abstract | PURPOSE: Maintenance of avascularity of the normal cornea and control of neovascularization during wound healing depend on a balance of angiogenic and antiangiogenic factors. The purpose of this paper is to determine the ability of corneal cells to convert plasminogen to angiostatins and to compare these products with those made by intact corneas. METHODS: RESULTS:
Plasminogen, its mRNA and angiostatins were found in human corneal tissue extracts from the epithelial, stromal, and endothelial layers and from cornea conditioned medium, but not in medium from cultured epithelial cells, stromal fibroblasts, or myofibroblasts. However, cultures of corneal epithelial cells and stromal fibroblasts were able to convert exogenously added plasminogen to angiostatins, whereas cultured myofibroblasts did not. Angiostatins of 38 and 34 kDa were found under all angiostatin generating conditions; however other angiostatins differed in size. Further, the angiostatins isolated from fibroblast culture supernatants inhibited vascular endothelial cell proliferation. CONCLUSIONS:
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Authors | Debra J Warejcka, Kimberly A Vaughan, Audrey M Bernstein, Sally S Twining |
Journal | Molecular vision
(Mol Vis)
Vol. 11
Pg. 859-68
(Oct 20 2005)
ISSN: 1090-0535 [Electronic] United States |
PMID | 16270025
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Messenger
- Angiostatins
- Plasminogen
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Topics |
- Angiostatins
(biosynthesis, genetics, pharmacology)
- Blotting, Western
- Cell Culture Techniques
- Cell Proliferation
(drug effects)
- Corneal Stroma
(cytology, drug effects, metabolism)
- Electrophoresis, Polyacrylamide Gel
- Endothelium, Vascular
(cytology)
- Epithelium, Corneal
(cytology, drug effects, metabolism)
- Fibroblasts
(metabolism)
- Humans
- Organ Culture Techniques
- Plasminogen
(genetics, metabolism, pharmacology)
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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