Association of MTRRA66G polymorphism (but not of MTHFR C677T and A1298C, MTRA2756G, TCN C776G) with homocysteine and coronary artery disease in the French population.

Abstract

Methylenetetrahydrofolate reductase polymorphism (MTHFR C677T) is an established determinant of homocysteine plasma level (t-Hcys) while its association with coronary artery disease (CAD) seems to be more limited. In contrast, the association of the substitutions A2756G of methionine synthase (MTR), A66G of methionine synthase reductase (MTRR) and C776G of transcobalamin (TCN) to both t-Hcys and CAD needs to be evaluated further. The objective was to evaluate the association of these polymorphisms with t-Hcys and CAD in a French population. We investigated the individual and combined effects of these polymorphisms and of vitamin B12 and folates with t-Hcys in 530 CAD patients and 248 matched healthy controls. t-Hcys was higher in the CAD group than in controls (11.8 vs 10.4 microM, P < 0.0001) and in carriers of MTRRAA and MTHFR 677TT than in those carrying the most frequent allele of both polymorphisms (13.8 vs 11.4 microM, P = 0.0102 and 12.5 vs 11.0 mM, P = 0.0065 respectively). The frequency of MTRR A allele was higher in CAD patients than in controls (0.48 [95% CI: 0.44-0.52] vs 0.38 [95% CI: 0.32-0.44], P = 0.0081) while no difference was observed for MTHFR 677T frequency. In multivariate analysis, t-Hcys > median and MTRRAA genotype were two significant independent predictors of CAD with respective odds ratios of 3.1 (95 % CI: 1.8-5.1, P < 0.0001) and 4.5 (95% CI: 1.5-13.1, P = 0.0051). In conclusion, in contrast to North Europe studies, MTRRAA genotype is a genetic determinant of moderate hyperhomocysteinemia associated with CAD in a French population without vitamin fortification.

• Authors: Rosa-Maria Guéant-Rodriguez, Yves Juilliére, Mirande Candito, Charles E Adjalla, Pierre Gibelin, Bernard Herbeth, Emmanuel Van Obberghen, Jean-Louis Gueánt
• Journal: Thrombosis and haemostasis (Thromb Haemost) Vol. 94 Issue 3 Pg. 510-5 (Sep 2005) ISSN: 0340-6245 [Print] Germany
• PMID: 16268464 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Transcobalamins
• Homocysteine
• Folic Acid
• methionine synthase reductase
• Ferredoxin-NADP Reductase
• Methylenetetrahydrofolate Reductase (NADPH2)
• 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
• Vitamin B 12

Topics

• 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase (genetics)
• Aged
• Alleles
• Case-Control Studies
• Coronary Artery Disease (blood, genetics)
• Female
• Ferredoxin-NADP Reductase (genetics)
• Folic Acid (blood)
• France
• Genetic Predisposition to Disease
• Genotype
• Homocysteine (blood)
• Humans
• Male
• Methylenetetrahydrofolate Reductase (NADPH2) (genetics)
• Middle Aged
• Polymorphism, Genetic
• Transcobalamins
• Vitamin B 12 (blood)
• White People